Percorrer por autor "Oliveira, Leandro Licursi"
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- Blueberry Extract and Resistance Training Prevent Left Ventricular Redox Dysregulation and Pathological Remodeling in Experimental Severe Pulmonary Arterial HypertensionPublication . Leite, Luciano Bernardes; Soares, Leôncio Lopes; Guimarães-Ervilha, Luiz Otávio; Costa, Sebastião Felipe Ferreira; Generoso, Sara Caco dos Lúcio; Xavier, Mirielly Alexia Miranda; Iasbik-Lima, Thainá; Oliveira, Leandro Licursi; Lucia, Ceres Mattos Della; Bianchi, Sara Elis; Bassani, Valquíria Linck; Herter, Flavio Gilberto; Turck, Patrick; Araujo, Alex Sander da Rosa; Forte, Pedro; Reis, Emily Correna Carlo; Machado-Neves, Mariana; Natali, Antônio JoséTo investigate whether the regular administration of blueberry extract and low-intensity resistance exercise training (RT), either alone or in combination, during the development of monocrotaline (MCT)-induced severe pulmonary arterial hypertension (PAH) in rats protect the left ventricle (LV) from redox dysregulation and pathological remodeling. Groups of seven male Wistar rats were formed for the experiment: sedentary control; sedentary hypertensive; sedentary hypertensive blueberry; exercise hypertensive; and exercise hypertensive blueberry. PAH was experimentally induced through a single intraperitoneal administration of MCT at a dose of 60 mg/kg. One day after injection, the blueberry groups started receiving a daily dose of blueberry extract (100 mg/kg) by gavage, while the exercise groups initiated a three-week program of RT (ladder climbing; 15 climbs carrying 60% of maximum load; one session/day; 5 times/week). Echocardiographic evaluations were conducted 23 days after injection, and the rats were euthanized the next day to harvest LV tissue. Separately, blueberry extract and RT mitigated augments in pulmonary artery resistance, LV tissue redox dysregulation (i.e., increased PC levels) and detrimental remodeling (i.e., reduced inflammation), and reductions in ejection fraction (EF) and fractional shortening (FS) caused by PAH. The combination of treatments prevented reductions in EF and FS, along with the development of a D-shaped LV. blueberry extract and moderate-intensity resistance training administered during the development of MCT-induced severe PAH in rats prevented LV redox dysregulation and pathological remodeling, thereby preserving its function.
- Combined nandrolone and resistance training induced cardiac remodelling and oxidative stress despite enhanced cardiomyocyte contractilityPublication . Moraes, Alexa Alves de; Suarez, Pedro Zavagli; Quintão, Arthur Eduardo de Carvalho; Fontes, Beatriz Lana; Costa, Sebastião Felipe Ferreira; Rocha, Carolina Camargos; Soares, Leôncio Lopes; Leite, Luciano Bernardes; Oliveira, Leandro Licursi; Reis, Emily Correna Carlo; Oliveira, Edilamar Menezes de; Forte, Pedro; Natali, Antônio José; Carneiro-Júnior, Miguel AraújoNandrolone decanoate (ND) is widely used by individuals engaged in resistance training (RT), yet their combined effects on cardiac function remain unclear. Objective To investigate the effects of RT and ND on cardiac structure and function, cellular contractility, Ca-2 (+) -handling protein expression, and redox balance in rats. Methods Thirty-two male Wistar rats were assigned to four groups: control (C), trained (C-T), ND (N), and trained ND (N-T). Animals received ND or saline for eight weeks, and RT was performed 3 x /week. Cardiac function was assessed by echocardiography, and isolated cardiomyocytes from the left and right ventricles (LV and RV) were evaluated for contractile function. Protein expression of Ca-2 (+) -handling regulators and oxidative stress markers was quantified. Results N-T increased LV and RV diameters by 25% and 33%, septal thickness by 41.7%, and reduced ejection fraction by 12.3% compared to N (p < 0.05). LV cardiomyocytes increased width by 29% and volume by 23% in N-T versus C-T (p <= 0.04). In LV, N-T showed greater contraction amplitude and velocity at 5 Hz (p = 0.04) and increased relaxation velocity at 1, 3, and 5 Hz (p < 0.01), with shorter time to peak contraction and 50% relaxation at 1 Hz versus N (p = 0.04). In RV, N-T increased contraction amplitude and velocity at 1 and 5 Hz, reduced time to peak at 1 and 3 Hz, shortened relaxation time at 1 Hz, and showed higher relaxation velocity than N and C-T (p < 0.02). Molecular analyses revealed reduced RyR2 expression (25% in LV and 9% in RV) and a 41% decrease in LV phospholamban in N-T versus C-T (p < 0.05). N-T also exhibited higher LV malondialdehyde compared to C-T (p = 0.03). Conclusion ND combined with RT induced adverse cardiac remodeling and impaired ventricular function, despite enhanced cardiomyocyte contractility, and intensified molecular and oxidative disturbances, indicating a maladaptive cardiac response. This is the first study to demonstrate regional differences in contractility and redox balance of isolated ventricular cardiomyocytes under ND plus RT.
- Resistance Training Before Hyperalgesia Induction Promotes Analgesic Effects Through Central and Peripheral Biomarker Modulation in an Experimental Fibromyalgia-like ModelPublication . Chiapeta, Andrês Valente; Oliveira, Leandro Licursi; Leite, Luciano Bernardes; Silva, Bruna Aparecida Fonseca da; Costa, Sebastião Felipe Ferreira; Soares, Leôncio Lopes; Moraes, Alexa Alves de; Drummond, Lucas Rios; Forte, Pedro; Natali, Antônio José; Carneiro-Júnior, Miguel AraújoFibromyalgia is a chronic syndrome characterized by widespread pain and complex pathophysiology, requiring new therapeutic approaches. This study aims to investigate the effects of resistance training (RT) before hyperalgesia induction on pain sensitivity, IL-6 and IL-10 expression in skeletal muscle, and thalamic serotonin levels in a fibromyalgia (FM)-like rat model. Methods: Wistar female rats aged 12 months were divided into four groups: untrained neutral saline (UNS), untrained acid saline (UAS), RT neutral saline (RTN), and RT acid saline (RTA). Both the RTN and RTA groups were subjected to an RT protocol comprising vertical ladder climbing three times a week throughout 14 weeks. The UAS and RTA groups received 100 mu L of neutral, sterile saline (pH 4.0) in the left gastrocnemius muscle, while the UNS and RTN groups received 100 mu L of neutral saline (pH 7.4). Mechanical hyperalgesia was assessed using Von Frey's electronic esthesiometer. Expression of interleukins 6 (IL-6) and 10 (IL-10) was analyzed in skeletal muscle, and serotonin expression was quantified in the thalamus. Results: After hyperalgesia induction, the RT groups demonstrated higher paw withdrawal thresholds than the UAS group (p < 0.05). Both IL-6 and IL-10 expression was lower in the RTA group compared to the UAS group (p < 0.05). Thalamic serotonin expression was higher (p < 0.05) in the RTA group compared to the UAS group. Conclusion: Previous RT was able to prevent mechanical hyperalgesia experienced by rats after its induction by acid saline by preventing the increase in the IL-6 and IL-10 levels in skeletal muscle and preventing the decrease in thalamic serotonin expression.