Percorrer por autor "Nunes, M. Eugénia"
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- Diversity assessment in the Portuguese genebank collection of tomato (Solanum lycopersicum L.) accessions: Insights for breeding and sustainable conservationPublication . Pinela, José; Montoya, César; Martins, Valter; Nunes, M. Eugénia; Rocha, Filomena; Lopes, Violeta R.; Barata, Ana Maria; Carvalho, Ana Maria; Ferreira, Isabel C.F.R.; Barros, LillianTomato germplasm is source of variability used by plant breeders to develop improved cultivars and part of the natural heritage to be preserved. In this study, the diversity among twenty Portuguese tomato accessions was evaluated, considering morphological and agronomic descriptors, molecular data, and compositional aspects. The tomato accessions captured 77.5% of the total 19 alleles detected. Important information on the selection of SSR loci in diversity analysis for the tomato genotypes was provided. The studied genotypes were rich in car-bohydrates and source of ascorbic acid. Citric acid and palmitic, linoleic, oleic, stearic, and alpha-linolenic acids were the major organic and fatty acids, respectively. The four tocopherol isoforms were detected and alpha-tocopherol was the most abundant. When correlating molecular and compositional data, it seemed that the greater the het-erozygosity, the greater the compositional variance. However, despite the genetic distance that characterized these accessions, some were chemically similar. Overall, the genetic differentiation among genotypes provided useful information for parental selection in breeding and other genetic studies.
- HRM analysis targeting ITS1 and matK loci as potential DNA mini-barcodes for the authentication of Hypericum perforatum and Hypericum androsaemum in herbal infusionsPublication . Costa, Joana; Campos, Bruna; Amaral, Joana S.; Nunes, M. Eugénia; Oliveira, Beatriz; Mafra, IsabelHypericum species are among the medicinal plants that are being increasingly used due to their reported health benefits. Hypericum perforatum (St. John's wort) is well known for its anti-inflammatory, antidepressive and anti-viral properties, as well as a healing agent. In Portuguese folk medicine, Hypericum androsaemum (Hiperic~ao do Ger^es) is largely used for its hepatic protective and diuretic properties. This work aimed at searching the potential use of two DNA mini-barcode candidates (ITS1 and matK) for the authentication of H. perforatum and H. androsaemum in herbal infusions. The sequencing results from ITS1 and matK regions were the basis for the development of species-specific PCR and real-time PCR assays coupled to High Resolution Melting (HRM) analysis, as simple approaches for the reliable discrimination of both species. The barcode regions were successful in the species-specific PCR identification of each target. ITS1 region revealed some intra-species variability from sequencing results, which compromises HRM analysis, while matK showed to be an adequate mini-barcode for the differentiation of both species by real-time PCR coupled to HRM analysis. The assays were effectively applied to commercial herbal infusions, enabling the consistent identification of two labels with non-declared Hypericum species.