Browsing by Author "Moreira, Maria Eduarda Soares Tofolli"
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- Alginate-based film blended with propolis loaded sporopollenin exine microcapsules: unveiling the potential of natural substances for wound healingPublication . Moreira, Maria Eduarda Soares Tofolli; Falcão, Soraia; Ribeiro, Maria Carolina; Aylanc, VolkanHealing a wound is one of the complex biological processes associated with tissue growth and regeneration, controlled by various biochemical and cellular mechanisms. In recent years, the creation of dressings using biopolymer-based materials with natural extracts has gained prominence. With this in mind, the ideal dressing should promote a moist environment while acting as a barrier against microorganisms. Its composition should include non-toxic, non-allergenic, and adherent substances, being essential to ensure easy removal and high effectiveness in protecting the wound against bacterial infections. Moreover, dressings should possess anti-inflammatory properties while promoting the overall healing process. This study explores the impact of extracts from two types of propolis on wound healing: red propolis and brown propolis. To produce the dressing, an alginate-based film was created, and propolis was incorporated into its composition through the vacuum-loading method in sporopollenin microcapsules, which are derived from bee pollen grains of Cytisus Shrub Species (Cytisus spp.). This incorporation into the film is essential for the controlled release of bioactive compounds into the wound. Spectrophotometric analyses determined that brown propolis achieved better inhibition results, which align with FTIR assays, where a higher composition of phenolic acids was observed in brown propolis samples compared to red propolis. Brown propolis films showed higher antioxidant activity (76.62% inhibition), outperforming red propolis (60.55%). The encapsulation efficiency of the microcapsules ranged from 67.35% to 74.48%, while the release rate of phenolic compounds reached up to 75% within 48 hours. Cytotoxicity tests indicated cell viability below 20% after 24 hours, evidencing toxicity at high concentrations. Therefore, it is noted that, for cellular analyses, the results were not satisfactory given that the dressings showed to be toxic to the cells because of the high value of propolis incorporated. Despite of these results, it is possible to conclude that the dressings have great potential as they are full of antioxidants and antibacterial substances. For better results less propolis and loaded SECs should be incorporated into the films. Ultimately, it was possible to develop a dressing with satisfactory quality, although broader studies are necessary for its practical use.