Browsing by Author "Mafra, Marcos R."
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- Effect of Curcuma longa L. extract and curcumin on porcine pancreatic α-amylase structure and activityPublication . Oliveira, Grazielle; Kaspchak, Elaine; Oliveira, Anielle; Leimann, Fernanda Vitória; Philippsen, Gisele Strieder; Seixas, Flávio Augusto Vicente; Igarashi-Mafra, Luciana; Mafra, Marcos R.This work aimed to evaluate alpha-amylase activity and structure in the presence of Curcuma longa L. extracts obtained with deep eutectic solvent (DES) and curcumin. Isothermal titration calorimetry (ITC), fluorescence, circular dichroism (CD), and molecular docking were applied to study the effect of additives on enzyme activity and structure. Results showed that in the presence of turmeric extracts, there was a lower catalytic rate. Through the ITC analysis, a lower reaction rate was noticed, related to the inhibition of the enzymatic activity, both in the presence of the extract and of curcumin. The turmeric extracts interacted with the enzyme by a static mechanism as demonstrated by fluorescence. CD showed an increase in negative bands characteristic of hydrophobic interactions between the enzyme and the samples, which probably difficult the access of substrate to the enzyme's active site. Molecular docking showed that curcumin is capable to interact with the active site of alpha-amylase confirming results obtained by other techniques. Results presented in this work show the turmeric extracts potential for alpha-amylase inhibition which may be of interest to the pharmaceutical and food industry.
- Extraction of bioactive compounds from Curcuma longa L. using deep eutectic solvents: in vitro and in vivo biological activitiesPublication . Oliveira, Grazielle; Marques, Caroline; Oliveira, Anielle; Santos, Amanda de Almeida; Amaral, Wanderlei; Ineu, Rafael P.; Leimann, Fernanda Vitória; Peron, Ana Paula; Igarashi-Mafra, Luciana; Mafra, Marcos R.In this work, deep eutectic solvents (DES-based menthol and cholinium chloride) and the ethanol, temperature, and times were selected to extract bioactive compounds from the rhizome, leaves, and flowers Curcuma longa L., using ultrasound-assisted extraction. Analyzes antioxidant, flavonoids, antimicrobial, chelation Fe2+, inhibition of the cholinesterase's enzymes, cytotoxicity, and genotoxicity in Allium cepa cells were performed. The extracts showed results of iron chelation and antibacterial. Curcuma flowers and leaves' extracts inhibited food spoilage bacteria with values above 45%, with substantial iron-chelating activity above 50%. Extracts obtained by DES based on menthol and lactic acid exhibited a high percentage of inhibition of acetyl and butyryl cholinesterase. In contrast, flower extracts obtained by menthol and acetic acid showed low inhibition of cholinesterase enzyme activity. No extract showed cytotoxicity and genotoxicity. Biological activities showed a high potential for the application of these extracts in the food and pharmaceutical industries.