Percorrer por autor "Landim, Euclides"
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- Côa Valley’s medicinal plants as potential cosmetic ingredients: cytotoxic and antioxidant assessmentPublication . Marques, Mário Pedro; Landim, Euclides; Varela, Carla; Marques, Joana; Costa, Ricardo M.F. da; Carvalho, Luís A.E. Batista de; Carvalho, Aida; Oliveira, Paulo J.; Cabral, CéliaWhere is located and what is the importance of river Côa Valley? • The river Côa Valley is part of the Guarda District, in the Northeast of Portugal. The Valley comprises an Archeological Park, which is considered “the most important open-air Paleolithic rock art site” in the world, being classified as UNESCO World Heritage Site since 1998. What are the natural resources’ relevance of this region? • The flora of the Archeologic Park in river Côa Valley comprises approximately 500 to 600 different plant species, some Portuguese and/or Iberian endemic species, according to a botanical survey recently carried out by us. Even though, so far, little is known about the natural endogenous resources of this territory, namely concerning medicinal plants. What is the aim of this investigation? • The main aim is to assess the cytotoxicity in skin fibroblasts (NHDF cell line) and the antioxidant activity through cell-free methods, of hydroalcoholic extracts obtained from selected plant species (Figure 1). These extracts are meant to be incorporated into scientific-validated plantbased cosmetic formulations, hence creating an exclusive cosmetic brand for Côa Valley.
- Côa Valley’s medicinal plants as potential cosmetic ingredients: cytotoxic and antioxidant screeningPublication . Marques, Mário Pedro; Landim, Euclides; Varela, Carla; Marques, Joana; Costa, Ricardo; Carvalho, Luís de; Carvalho, Aida; Oliveira, Paulo; Cabral, CéliaCôa Valley is a Northeast region of Portugal, classified as a UNESCO World Her- itage Site since 1998, and recognised as “the most important open-air Paleo- lithic rock art site”. So far, little is known about the natural endogenous resourc- es of this territory, namely medicinal plants. According to a preliminary ethnobotanical survey in this region, several medicinal plants were mentioned to have skin-beneficial effects. However, many of them still lacking scientific validation. Taking into account the results gathered during the survey carried in the CôaMedPlants project, the main goal of this investigation is to assess the cytotoxicity and antioxidant activity of selected species to incorporate them into scientific-validated plant- based cosmetic formulations and create an ex- clusive cosmetic brand for Côa Valley. Therefore, nine species were selected and their hydroalcoholic extracts (EtOH 80 %) were prepared. Their non-cyto- toxic concentrations were determined in vitro using the Normal Human Dermal Fibroblasts (NHDF) cell line, by the evaluation of metabolic activity through the Alamar Blue assay, and cell mass estimation according to the sulforhodamine B (SRB) assay. Most of the extracts revealed non-toxic concentrations ≤ 0.2 mg/mL using the NHDF cell model. Afterwards, non-cellular techniques were used to screen the antioxidant activity of these ethanolic extracts, through DPPH, ABTS, CUPRAC and FRAP assays. From the 9 species studied, the most promising so far are: Arbutus unedo L., Cistus albidus L., Cistus salviifolius L., Lavandula pedunculata (Mill.) Cav. and Pistacia terebinthus L., which will be further investigated in- depth, to characterise their antioxidant potential using in vitro cell models.
- A Spectrochemically Driven Study: Identifying Phenolic-Rich Extracts from Helichrysum stoechas, Lavandula pedunculata, and Thymus mastichina with Potential to Revert Skin Aging EffectsPublication . Marques, Mário Pedro; Landim, Euclides; Varela, Carla; Costa, Ricardo M. F. da; Marques, Joana; Carvalho, Luís A. E. Batista de; Silva, Ana; Cruz, Maria Teresa; André, Rebeca; Rijo, Patrícia; Dias, Maria Inês; Carvalho, Aida; Oliveira, Paulo J.; Cabral, CéliaPlants inhabiting mediterranean-influenced climatic zones, like Helichrysum stoechas (L.) Moench subsp. stoechas, Lavandula pedunculata (Mill.) Cav., and Thymus mastichina (L.) L. subsp. mastichina, have been scarcely investigated regarding their richness in phenolic compounds, herein explored as sources of skin anti-aging compounds. In this investigation, Fourier transform infrared spectroscopy (FTIR) in attenuated total reflectance (ATR) mode and high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) were employed to chemically characterize the hydroethanolic extracts (HEs), and their cell-free antioxidant potential was screened. Thereafter, non-toxic concentrations of HEs were determined in human skin cells using Alamar blue® and Sulforhodamine B assays. The cytoprotective and antioxidant effects of HEs were assessed in tert-butyl hydroperoxide-stimulated fibroblasts, their anti-inflammatory potential was studied in lipopolysaccharide-injured macrophages, and enzymatic inhibition assays were performed. Notably, the irritant effects of HEs were tested according to Test Guideline No. 439 of the Organization for Economic Co-operation and Development (OECD). The major compounds identified in the T. mastichina and L. pedunculata HEs were rosmarinic and salvianolic acid derivatives, while H. stoechas HE was mainly composed of caffeoyl and feruloyl derivatives, and O-glycosylated flavonoids. T. mastichina (≤0.4 mg/mL) exhibited significant cytoprotective, anti-inflammatory, and antioxidant effects, as well as remarkable anti-hyaluronidase activity. Shedding light on the quantitative and qualitative chemical picture of these HEs highlighted T. mastichina as a promising candidate to target skin aging effects, which correlates with its phenolic content. Further investigation is warranted regarding its anti-aging pharmacological activity, which could lead to the development of plant-based skin anti-aging products.