Percorrer por autor "Júlio, Ana Carolina"
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- Controlled-release and stabilized fertilizers are equivalent options to split application of ammonium nitrate in a double maize-oats cropping systemPublication . Arrobas, Margarida; Chiocheta Junior, José Carlos; Damo, Letícia; Júlio, Ana Carolina; Hendges, Igor Pereira; Wagner, Américo; Godoy, Wilson Itamar; Cassol, Luís César; Rodrigues, M.A.The application of fertilizers as a topdressing in maize raises serious concerns because too much fertilizer is retained in the upper leaves, causing burning to the tissues. In this study, the use of a controlled-release and a stabilized fertilizer (with 3, 4-dimethylpyrazole phosphate) was compared with the application of a conventional fertilizer split into two equivalent applications in a forage maize-oats cropping system. In maize, 100 and 200 kg N ha−1 of different fertilizers were used in addition to an unfertilized control. The oat crop was not fertilized, since it served only as a winter catch crop. Maize dry matter (DM) yield increased significantly with N rate only in 2019, being the second growing season, with the control showing the lowest average value (7.1 t ha−1). The most fertilized treatments (200 kg N ha−1) gave the highest DM yields, ranging between 14.2 and 16.7 t ha−1, but with no significant differences between them. Oats had a relevant role as a catch crop recovering residual N that could have potentially been lost from the soil. Stalk nitrate concentration proved to be very sensitive to N fertilization (varying from 150.4 to 1945.6 mg kg−1 in 2018 and 494.9 to 1574.9 mg kg−1 in 2019), showing great potential as a tool of N management. These three fertilization strategies seem to be valid options that farmers can consider, after incorporating technical-economic information related to equipment suitability and the price of fertilizers.
- Roots and rhizomes of wild Asparagus: Nutritional composition, bioactivity and nanoencapsulation of the most potent extractPublication . Adouni, Khawla; Júlio, Ana Carolina; Santos-Buelga, Celestino; González-Paramás, Ana María; Filipe, Patrícia; Rijo, Patrícia; Lima, Sofia A. Costa; Reis, Salette; Fernandes, Ângela; Ferreira, Isabel C.F.R.; Fernández-Ruiz, Virginia; Morales, Patricia; Flamini, Guido; Achour, Lotfi; Fonte, PedroThe nutritional composition and bioactive properties of roots and rhizomes of Asparagus stipularis were evaluated. Antioxidant activity of extracts obtained by infusion was evaluated using free radicals scavenging and reducing power methods. Porcine liver primary cell was used to check the hepatotoxicity of infusions. Results revealed that Asparagus samples are likely a source of nutrients, such as dietary fibre and essential fatty acids. HPLC-DAD-ESI/MS characterization of infusions allowed the identification and quantitation of 7 phenolic compounds, all hydroxycinnamoyl derivatives, with caffeic acid as the most abundant. Roots infusion contained the highest amounts of these compounds. It also exhibited the highest antioxidant activity in all assays, with EC50 values of 0.44 ± 0.01, 0.98 ± 0.03 and 0.64 ± 0.01 mg/mL for DPPH, ABTS and FRAP assays, respectively, with no toxicity towards PLP2 primary cell cultures (GI50 > 400 μg/mL). PLGA nanoparticles loaded with root extract were prepared using solvent-evaporation double emulsion method. Nanoparticles size was about 260 nm and a polydispersity index around 0.1, with a zeta potential of about -36 mV, as well as a good encapsulation efficiency of approximately 83%. Their morphology was analysed by SEM and spherical polymeric nanoparticles with a smooth surface were observed. FTIR and DSC were also performed, which allowed corroborating the efficacy of the encapsulation and to confirm the production of a stable and robust system to load Asparagus extracts. The developed nanoparticles are expected to be used as delivery systems for bioactive compounds of A. stipularis and they could be used as an innovative dietary supplement.