Percorrer por autor "Galindo-Rosales, Francisco J."
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- Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contractionPublication . Calejo, Joana A.C.; Pinho, Diana; Galindo-Rosales, Francisco J.; Lima, Rui A.; Campo-Deaño, LauraThe interest in the development of blood analogues has been increasing recently as a consequence of the increment in the number of experimental hemodynamic studies and the difficulties associated with the manipulation of real blood in vitro because of ethical, economical or hazardous issues. Although one-phase Newtonian and non-Newtonian blood analogues can be found in the literature, there are very few studies related to the use of particulate solutions in which the particles mimic the behaviour of the red blood cells (RBCs) or erythrocytes. One of the most relevant effects related with the behaviour of the erythrocytes is a cell-free layer (CFL) formation, which consists in the migration of the RBCs towards the center of the vessel forming a cell depleted plasma region near the vessel walls, which is known to happen in in vitro microcirculatory environments. Recent studies have shown that the CFL enhancement is possible with an insertion of contraction and expansion region in a straight microchannel. These effects are useful for cell manipulation or sorting in lab-on-chip studies. In this experimental study we present particulate Newtonian and non-Newton ian solutions which resulted in a rheological blood analogue able to form a CFL, downstream of a microfluidic hyperbolic contraction, in a similar way of the one formed by healthy RBCs.
- Visualization of PMMA blood analogue fluid flowing through a hyperbolic microchannelPublication . Calejo, Joana A.C.; Pinho, Diana; Garcia, Valdemar; Galindo-Rosales, Francisco J.; Lima, Rui A.; Campo-Deaño, LauraAxial migration of individual red blood cells (RBCs) is a well known effect that happens in microchannels with dimensions below 300 μm. This effect is known as the Fåhræus-Lindqvist effect gives place to a cell free layer (CFL) of plasma located around the walls of the microchannel [1]. Several flow studies regarding the CFL measurement exist in in vitro [1-3] environments. However, the manipulation of in vitro blood is often difficult due to the ethical, economical and safety issues involved. As a result, the development of blood analogue solutions is needed to improve our understanding regarding the blood flow behaviour in microchannels and microvessels. Although several studies using one-phase Newtonian and non-Newtonian blood analogues can be found in the literature [4, 5], there are few works related with the use of particulate solutions in which the particles mimic the RBCs behaviour. The present work proposes a particulate-viscoelastic solution made of xanthan gum and dextran with rigid polymethylmethacrylate (PMMA) spherical particles able to mimic the effect of CFL formation that frequently happens in in vitro blood flow systems. Flow visualizations through hyperbolic contraction microchannels were used to observe the CFL originated by the proposed blood analogue fluid. Additionally, this CFL was compared with the plasma layer formed by an in vitro blood sample.