Browsing by Author "Fonte, Elizabete"
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- Ação do ácido quetomélico nas alterações hematológicas induzidas pela redução de 5/6 de massa renal em ratosPublication . Fonte, Elizabete; Nogueira, António José M.; Pires, Carlos André; Colaço, Bruno Jorge; Oliveira, Paula A.; Pires, Maria JoãoA doença renal crónica está associada com o desenvolvimento de anemia devido principalmente à diminuição da produção de eritropoetina. O ácido quetomélico (AC) é um potente inibidor seletivo da farnesil transferase e, desta forma, inibe as vias de sinalização que levam à progressão da fibrose renal. Assim, o objectivo deste trabalho foi o de estudar os efeitos do tratamento crónico com o AC nas alterações hematológicas induzidas pela redução de 5/6 de massa renal (RMR). Neste estudo foram utilizados 70 ratos machos da estirpe Wistar. Uma semana após a redução cirúrgica de 5/6 de massa renal, os animais foram divididos em quatro grupos experimentais: CO: animais em que se simulou a cirurgia e sem tratamento; CO+AC: animais em que se simulou a cirurgia e tratados com o AC; RMR: animais com RMR e não tratados; RMR+AC: animais com RMR e tratados com o AC. O tratamento foi administrado por via intraperitoneal, durante seis meses. Para o estudo hematológico procedeu-se à contagem manual, em câmara de Neubauer, do número total de eritrócitos e de leucócitos, à determinação do microhematócrio, à contagem diferencial de leucócitos e à estimativa do número total de plaquetas no esfregaço de sangue corado com Diff-Quick. Nos grupos RMR e RMR+AC observou-se um aumento estatisticamente significativo (p<0.01) do número total de eritrócitos, do microhematócrito, do número total de leucócitos e das plaquetas, em relação aos grupos CO e CO+AC. Deste modo, os resultados deste estudo sugerem que em ratos Wistar com doença renal crónica, o tratamento com o AC, durante seis meses, não diminui a gravidade da anemia, da leucocitose e da trombocitose induzidas pela redução de 5/6 de massa renal.
- Effect of chaethomellic acid on renal function in a rat model of chronic renal failurePublication . Nogueira, António José M.; Fonte, Elizabete; Oliveira, Paula A.; Colaço, Bruno Jorge; López-Novoa, Jose; Mega, Carmén; Colaço, Aura; Pires, Maria JoãoTo study the effect of chronic treatment with chaethomellic acid (CA), a highly specific inhibitor of ras farnesyl-protein transferase, on the renal function of rats with renal failure induced by renal mass reduction. Male Wistar rats were subjected to 5/6 nephrectomy (RMR) or sham-operated (SO). One week after surgery, rats have been placed in four experimental groups: RMR: rats without treat- ment (n=13); RMR+CA: rats treated with CA (n=13); SO: rats without treatment (n=13); SO+AC: rats treated with CA (n=13). CA was intraperitoneally administered in a dose of 0.23 g/Kg three times a week for 6 months. Creatinine, blood urea nitrogen (BUN) and protein were measured in serum and/or urine by routine laboratory techniques. BUN, creatinine, and proteinuria were significantly lower and creatinine clearance was significantly higher in SO and SO+AC groups when compared with RMR and RMR+AC groups. There were no differ- ences in creatinine, proteinuria and creatinine clearance between RMR and RMR+AC groups. Anyway, RMR+AC group showed significant lower BUN and lower creatinine and proteinuria, and higher creatinine clearance than RMR group. In a model of renal failure induced by RMR, 6 months of treatment with CA may have some beneficial effect on renal function.
- Effect of chaethomellic acid on renal function in rat model of chronic renal failurePublication . Nogueira, António José M.; Mega, Carmén; Fonte, Elizabete; Oliveira, Paula A.; Colaço, Bruno Jorge; López-Novoa, Jose; Colaço, Aura; Pires, Maria JoãoTo study the effect of chronic treatment with chaethomellic acid (CA), a highly specific inhibitor of ras farnesyl-protein transferase, on the renal function of rats with renal failure induced by renal mass reduction. Male Wistar rats were subjected to 5/6 nephrectomy (RMR) or sham-operated (SO). One week after surgery, rats have been placed in four experimental groups: RMR: rats without treat- ment (n=13); RMR+CA: rats treated with CA (n=13); SO: rats without treatment (n=13); SO+AC: rats treated with CA (n=13). CA was intraperitoneally administered in a dose of 0.23 g/Kg three times a week for 6 months. Creatinine, blood urea nitrogen (BUN) and protein were measured in serum and/or urine by routine laboratory techniques. BUN, creatinine, and proteinuria were significantly lower and creatinine clearance was significantly higher in SO and SO+AC groups when compared with RMR and RMR+AC groups. There were no differ- ences in creatinine, proteinuria and creatinine clearance between RMR and RMR+AC groups. Anyway, RMR+AC group showed significant lower BUN and lower creatinine and proteinuria, and higher creatinine clearance than RMR group. In a model of renal failure induced by RMR, 6 months of treatment with CA may have some beneficial effect on renal function.