Browsing by Author "Faria, Susana"
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- Determination of neutrophil FC receptor IIIB antigens (HNA-1A, HNA-1B AND HNA1C) by ploymerase chain reaction with sequence-specific fluorochrome-labeled primersPublication . Costa, Elísio; Antunes, Marika Bini; Faria, Susana; Vieira, Emília; Branca, Rosa; Barbot, José; Santos, Rosário
- Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reactionPublication . Costa, Elísio; Antunes, Marika Bini; Faria, Susana; Vieira, Emília; Branca, Rosa; Barbot, José; Santos, RosárioWe evaluate a technique for genotyping HNA-1a, -1b and -1c antigens, resorting to fluorescence-primed allele-specific polymerase chain reaction (FPAS-PCR), and determine the frequency of the different genotypes in a normal Portuguese population. Our results indicate that the FPAS-PCR system is a reliable and simple tool for genotyping the neutrophil Fcc receptor IIIB antigens. The HNA-1a, -1b and -1c gene frequencies of 42.98, 84.21 and 6.14%, respectively, found in this study are similar to those reported for other white populations.