Percorrer por autor "Costa, Eduardo M."
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- Fortification of yogurt with Solanum melongena phenolic extract: Physicochemical stability, bioaccessibility, bioavailability, and antioxidant effectsPublication . Añibarro-Ortega, Mikel; Gómez-García, Ricardo; Machado, Manuela; Pereira, Alexis; Costa, Eduardo M.; Pintado, Manuela; Barros, Lillian; Pinela, JoséThis study investigated the impact of fortifying yogurt with Solanum melongena phenolic extract by assessing its physicochemical, biochemical, and antioxidant stability, including its behavior during in vitro simulated digestion. Fortification influenced the yogurt's pH, titratable acidity, and color, and improved its cohesiveness during shelf life. The fortified yogurt exhibited higher glucose levels and reduced lactic acid production compared to the control. During simulated digestion, the extract modulated alterations in the protein and fatty acid profiles of the yogurt. Among phenolic compounds, 3-O-caffeoylquinic acids exhibited good stability during the oral and gastric phases but decreased in the intestinal digest. Some antioxidant effects were enhanced during digestion, particularly against lipid peroxidation, but declined during storage. Furthermore, phenolic acids were absorbed in an in vitro Caco-2 cell model, supporting their bioavailability. Overall, yogurt fortification with S. melongena extract improved nutritional and functional properties, highlighting its potential as a sustainable functional food. Future studies should address sensory acceptance, safety aspects, and in vivo health outcomes.
- Valorization of Solanum melongena L. crop by-products: Phenolic composition and in vitro antioxidant, antidiabetic, anti-inflammatory, cytotoxic, and antimicrobial propertiesPublication . Añibarro-Ortega, Mikel; Dias, Maria Inês; Petrović, Jovana; Núñez, Sonia; Calhelha, Ricardo C.; Costa, Eduardo M.; Machado, M.; Pintado, Manuela; Soković, Marina; López, Víctor; Barros, Lillian; Pinela, JoséThis study explored the valorization of post-harvest eggplant aerial parts as a sustainable source of value-added ingredients by investigating their phenolic composition and in vitro bioactive properties. HPLC-DAD-ESI/MSn analysis identified chlorogenic acid derivatives as the predominant phenolic compounds (53 % of the phenolic fraction), followed by O-glycosylated kaempferol and quercetin. The extract displayed antioxidant activity in physiologically relevant cell-based assays and significant α-glucosidase inhibitory capacity that far exceeded that of the standard drug acarbose. It also inhibited the formation of advanced glycation end-products (AGEs), suggesting its potential to mitigate diabetes-related complications. Furthermore, the extract showed a modest pancreatic lipase inhibitory effect and capacity to suppress interleukin 6 production. Selective cytotoxicity against human gastric and colon adenocarcinoma cell lines and strong antimicrobial activity against foodborne pathogens were observed. Given the growing demand for natural alternatives to synthetic drugs, these findings position eggplant crop biomass as a promising, sustainable source of active compounds with potential applications in food, nutraceutical, and pharmaceutical formulations for managing type 2 diabetes and other oxidative stress-mediated conditions. This study not only contributes to the valorization of agricultural waste but also expands the research on by-products of Solanaceae crops, offering a pathway for sustainable resource utilization.