Browsing by Author "Costa, Bruno"
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- Bee propolis effect on protection of RBC’s membrane integrityPublication . Moreira, Leandro; Rogão, Mónica; Pereira, Ana Paula; Morais, Margarida; Costa, Bruno; Dias, L.G.; Dias, Teresa; Estevinho, Leticia M.Propolis is a resinous substance collected from plants by bees. The propolis composition depends on the surrounding vegetation, the season, and the area from which derive. This hive product usually contains a variety of chemical compounds such as polyphenols (flavonoids, phenolic acids and esters), steroids, and amino acids. The hereditary spherocytosis (HS) is a congenital hemolytic anemia, with origin in the modification of membrane proteins of erythrocytes, which leads to increased susceptibility to hemolysis and a decrease of the cell over-life. The HS is the most common red blood cell (RBC) membrane disorder in European Caucasians, with a prevalence of roughly 200-300 per 106, and to Japanese population 5.7-20.3 per 106. The aim of the present work was to determine if propolis extracts could affected the red cell membrane integrity and comparing the effect of two propolis extracts from different regions (Bornes - Trás-os-Montes; Fundão - Beira Interior). In this work, two adults were studied, one with the syndrome HS splenectomized and one healthy used as control. Diagnosis of HS was made by clinical features, identification of spherocytes on peripheral blood smears and abnormal osmotic fragility. The results show that the two propolis extracts affected the erythrocyte membrane fragility in both individuals (control and patient with HS).
- Bee propolis effect on protection of RBCs membrane integrityPublication . Moreira, Leandro; Rogão, Mónica; Pereira, Ana Paula; Morais, Margarida; Costa, Bruno; Dias, L.G.; Dias, Teresa; Estevinho, Leticia M.Propolis is a resinous substance collected from plants by bees. The propolis composition depends on the surrounding vegetation, the season, and the area from which derive. This hive product usually contains a variety of chemical compounds such as polyphenols (flavonoids, phenolic acids and esters), steroids, and amino acids. The hereditary spherocytosis (HS) is a congenital hemolytic anemia, with origin in the modification of membrane proteins of erythrocytes, which leads to increased susceptibility to hemolysis and a decrease of the cell over-life.
- Própolis: efeito na fragilidade osmótica da membrana eritrocitáriaPublication . Moreira, Leandro; Rogão, Mónica; Pereira, Ana Paula; Costa, Bruno; Pereira, Ermelinda; Morais, Margarida; Pombo, Graça; Estevinho, Leticia M.Própolis é uma substância resinosa colhida das plantas, pelas abelhas. A composição do própolis depende da vegetação envolvente, da estação do ano, e da área da qual provém. Este produto da colmeia contém normalmente, uma grande variedade de compostos químicos, tais como polifenóis (flavonóides, ácidos fenólicos e ésters), esteróides e aminoácidos. A esferocitose hereditária (EH) é um tipo de anemia de transmissão hereditária que resulta da presença de eritrócitos microcíticos e hipercromicas, esféricos e sem palidez central. Clinicamente, os indivíduos apresentam normalmente desde condições assintomáticas até anemias hemolíticas graves. Os objectivos deste estudo foram avaliar o efeito de dois extractos de própolis, oriundos de diferentes regiões (Bornes – Trás-os-Montes; Fundão – Beira Interior), na fragilidade osmótica da membrana eritrocitária em pacientes com Esferocitose Hereditária. Para avaliar o efeito do própolis este foi extraído com DMSO (Dimetilsulfóxido), e posteriormente testado no sangue de dois indivíduos, um com esferocitose hereditária e outro sem esta patologia. O sangue utilizado foi obtido por punção venosa com EDTA sódico. No indivíduo portador da doença procedeu-se aos testes de fragilidade osmótica, tendo-se verificado que ambos os própolis demonstraram efeito sob a membrana do eritrócito, no entanto o efeito do própolis oriundo de Bornes - Trás-os-Montes foi mais acentuado comparativamente com o do Fundão – Beira Interior. Estes resultados abrem portas a investigações futuras, no sentido de elucidar os mecanismos pelos quais o própolis altera a fragilidade da membrana eritrocitária.
- Red blood cells disease (hereditary spherocytosis): propolis effectPublication . Moreira, Leandro; Pereira, Ana Paula; Bento, Margarida; Costa, Bruno; Dias, L.G.; Dias, Teresa; Estevinho, Leticia M.Propolis is a resinous substance collected from plants by bees, used since the Egyptian civilization. The hereditary spherocytosis(HS) is a type of transmission of hereditary anemia.Clinically, individuals usually present from a symptomatic conditions to severe haemolytic anaemia. The objective of this study was to evaluate the effect of two propolis extracts, in the osmotic fragility of patient red blood cell (RBC) membrane with HS. It was found that propolis decreased the erythrocytes membrane fragility, beingth effect of Bornes propolis more pronounced. The obtained results suggest that in vitro, the membrane fragility may be increased under oxidative stress conditions inpatient RBC’s, and the protection effect of propolis is due to its antioxidant properties. These results open doors for future investigations in order to elucidate the mechanisms, and identify the most relevant compounds involved in the fragility of the erythrocyte membrane.