Percorrer por autor "Chelgham, Abdelhakim"
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- Anti-Proliferative, Anti-Inflammatory, In Vitro Wound Healing Potentials and Phenolic Content of Phlomis Crinita Cav. ExtractsPublication . Chelgham, Abdelhakim; Borges, Ana Margarida; Calhelha, Ricardo C.; Merouane, Abdelaziz; Noui, Abdallah; Saadi, AbdelkaderPhlomis crinita Cav. (P. crinita), a medicinal plant from the Lamiaceae family, is widely used as a natural remedy in traditional North African and Southern European folk medicine to treat lesions and burns through the application of its leaves This study aimed to investigate its biological activities, including antiproliferative, anti-inflammatory, and wound-healing properties. Hydromethanolic extracts were prepared from the leaves, flowers, and rhizomes. Phytochemical analysis determined total phenolic content (TPC) and flavonoid content (FC). Antiproliferative activity was evaluated using the sulforhodamine B (SRB) assay against five human tumor cell lines (AGS, Caco-2, HeLa, MCF-7, and NCI-H460), each derived from a carcinoma of a distinct organ. Anti-inflammatory activity was assessed by measuring nitric oxide production in LPS-stimulated RAW 264.7 macrophages. The wound-healing potential was evaluated using the scratch assay. Phytochemical analysis revealed significant variations in TPC and FC among the extracts, with the rhizome extract exhibiting the highest levels: TPC: 128.15 μg GAE/mg DW and FC: 46.91 μg QE/mg DW. All extracts demonstrated antiproliferative activity against the tumor cell lines, with the rhizome extract showing the most potent growth inhibition (GI50: 178.11 ± 4.10 μg/mL against AGS). The rhizome extract also exhibited the most potent inhibition of nitric oxide production (IC50: 152.42 ± 10.88 μg/mL) compared to the other extracts tested. In the wound-healing assay, leaf extract demonstrated comparable healing effects to Allantoin, a known wound-healing agent. Our findings support the notion that P. crinita can be considered a promising source of therapeutic bioactive compounds.
- Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentialsPublication . Chelgham, Abdelhakim; Saadi, Abdelkader; Merouane, Abdelaziz; Bensouıcı, Chawki; Cakmak, Yavuz Selim; Pires, Tânia C.S.P.Phlomis crinita Cav. (P. crinita), known as " Khayat el-djerah " in Algerian folk medicine, is used for wound healing and abdominal pain relief. This study assessed the phytochemical profile, phenolic content, antimicrobial activity against five Gram-negative and three Gram-positive clinical bacterial strains, as well as in vitro antioxidant activity of hydroethanolic extracts from leaves (HLE), flowers (HFE), and rhizomes (HRE) of P. crinita. Fifteen phenolic compounds such as four flavonoids, trans-cinnamic acid, six cinnamic acid derivatives, and four benzoic acid derivatives were identified for the first time in P. crinita by HPLC-DAD, with quantitative differences among the analyzed parts. HRE exhibited high levels of total phenolics (262.97 ± 16.2 μg GAE/mg DW) and flavonoids (71.87 ± 3.25 μg QE/mg DW), while HLE had the highest flavonols content (18.89 ± 5.12 μg QE/mg DW). All extracts demonstrated strong antioxidant properties. HLE exhibited the highest potency, with IC50 values of 15.46 ± 0.45 µg/mL (DPPH) and 11.71 ± 0.50 µg/mL (ABTS). HLE exhibited good reducing power (FRAP A0.5 = 40.07 ± 2.82 µg/mL), while HRE showed the best reducing power (Phenanthroline A0.5 = 7.88 ± 1.63 µg/mL). All extracts revealed broad-spectrum antibacterial effects, and HRE exhibited the most potent activity against Enterococcus faecalis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL. These results showed that P. crinita could be useful as source of bioactive compounds for pharmaceutical and food industry.