ESA - Artigos em Revistas Indexados à WoS/Scopus
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Browsing ESA - Artigos em Revistas Indexados à WoS/Scopus by Author "Afonso, Paulo"
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- Leishmania seroprevalence in dogs: comparing shelter and domestic communitiesPublication . Afonso, Paulo; Coelho, Ana Cláudia; Quintas, Helder; Cardoso, Luís MiguelCanine leishmaniosis (CanL) is a chronic, systemic, and often severe disease. The main causative agent of CanL is a protozoan parasite, Leishmania infantum, with phlebotomine sand flies acting as vectors. In Europe and other continents, L. infantum is also responsible for leishmaniosis in other animals, such as cats, horses, and humans. In Portugal, animal and human leishmaniosis is endemic, and high prevalence levels of infections and disease have been reported in dogs. There is a prejudice against stray animals and also those housed in shelters, assuming they have higher levels of infection with vector-borne pathogens, including L. infantum, when compared to domestic animals. In northeastern Portugal, serum samples were obtained from March to May 2022 in three shelters (n = 179) and thirteen veterinary clinics (n = 164), resulting in 343 dogs being analyzed for antibodies to Leishmania spp. by the direct agglutination test (DAT). The overall seroprevalence was 9.9%, with 15.2% seroprevalence in domestic dogs and 5.0% in the shelter ones (p = 0.003). The fact that shelter dogs had a lower seroprevalence could be explained by more regular veterinary care provided in shelters regarding preventive measures, including insecticides with an antifeeding effect, in comparison with domestic dogs.
- Timed artificial insemination in Serrana nanny-goats: the effects of vaginal specula and artificial insemination techniciansPublication . Quintas, Helder; Silva, Daiane; Afonso, Paulo; Álvaro, Armindo; Mateus, Óscar; Valentim, RamiroThe success of artificial insemination (AI) depends on the equipment used, especially regarding the facilitation of artificial insemination technicians’ work and the positioning of insemination guns in the genital tract of animals. This study aimed to evaluate how vaginal specula and artificial insemination technicians affected the fertility rates of timed artificially inseminated Serrana Transmontano goats (which are indigenous to Portugal). For this, 58 adult nanny-goats aged between three and nine years were used. They were reproductively controlled by a short progestogen treatment (FGA) (seven days) with equine chorionic gonadotropin (eCG). Timed cervical AI (43 hours after the end of the hormonal treatment) was performed with chilled semen. Nanny-goats were inseminated by two artificial insemination technicians (A vs. B) alternating two vaginal specula (Minitub vs. “Reyes”). Pregnancy was diagnosed by ultrasound 41 days later. About 98% of nanny-goats responded to our treatment with FGA and eCG. In total, 82.8% of nanny-goats were pregnant 41 days after AI. Neither vaginal specula (Minitub: 81.2% vs. “Reyes”: 84.6%) nor artificial insemination technicians (A: 82.8% vs. B: 82.8%) affected fertility rates. Thus, we conclude that neither the vaginal specula used in this research nor the artificial insemination technicians affected Serrana goat pregnancy rates.