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Title: Identification of anthocyanin pigments in strawberry (cv Camarosa) by LC using DAD and ESI-MS detection
Authors: Lopes-da-Silva, M.F.
Pascual-Teresa, Sónia de
Rivas-Gonzalo, J.C.
Santos-Buelga, Celestino
Keywords: Strawberry
Liquid chromatography-mass spectrometry
Issue Date: 2002
Publisher: Springer-Verlag
Citation: Silva, M.F. Lopes da; Pascual Teresa, Sónia de; Rivas Gonzalo, J.C.; Santos Buelga, Celestino (2002) - Identification of anthocyanin pigments in strawberry (cv Camarosa) by LC using DAD and ESI-MS detection. European Food Research and Technology. ISSN 1438-2377. 214, p. 248-253
Series/Report no.: 214;
Abstract: New high performance liquid chromatography (HPLC) conditions were developed for the separation of strawberry anthocyanins that provided a good resolution of peaks at a low flow rate compatible with the requirements of the mass spectrometry (MS) detector. A strawberry extract was fractionated by column chromatography and simple fractions containing basically anthocyanins were obtained, making their analysis by HPLC possible using on-line photodiode array detection and MS. Information on the identity of the major and some secondary anthocyanins in strawberry was obtained froth their retention characteristics, UV-visible spectra and mass spectra. The presence in strawberry of the previously reported cyanidin 3-glucoside, pelargonidin 3-glucoside, pelargonidin 3-rutinoside and pelargonidin 3-acetylglucoside was confirmed and cyanidin 3-rutinoside was identified in strawberry for the first time. Furthermore, cyanidin 3-malonyldiglucoside, pelargonidin 3-malylglucoside, a pelargonidin bioside and two possible pelargonidin 3-biosides acylated with acetic acid were also tentatively assigned.
Peer review: yes
ISSN: 1438-2377
Publisher Version: DOI: 10.1007/s00217-001-0434-5
Appears in Collections:PTV - Artigos em Revistas Não Indexados ao ISI/Scopus

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