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Title: Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii
Authors: Kandemir, Irfan
Pinto, M. Alice
Meixner, Marina D.
Sheppard, Walter S.
Keywords: mtDNA
Cytochrome oxidase I
Apis mellifera lamarckii
O lineage
Issue Date: 2006
Publisher: Genetics and Molecular Biology
Citation: Kandemir, Irfan; Pinto, M. Alice; Meixner, Marina D.; Sheppard, Walter S. (2006) - Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A. m. lamarckii. Genetics and Molecular Biology. ISSN 1415-4757. 29:4, p. 747-749
Abstract: Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial “O” lineage, rather than a diagnostic site for A. m. lamarckii.
Peer review: yes
ISSN: 1415-4757
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Appears in Collections:ARN - Artigos em Revistas Indexados ao ISI/Scopus

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