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Advisor(s)
Abstract(s)
The Iberian Peninsula comprises a diverse set of habitats. It was an important glacial refugium
during the Pleistocene and has served as a bridge for populations migrating between Africa
and Europe, resulting in a complex mix of ancestry and diversity. The Iberian honey bee (A.
m. iberiensis) is no exception and has been the subject of numerous incongruent population
genetic surveys. Recent mtDNA and SNP analyses indicate a steep northeastern-southwestern
cline of African ancestry along the peninsula, which has been explained by selection.
Advances in DNA sequencing technology and computational tools provide unprecedented
opportunities to study demography, search for signatures of selection across the genome and
illuminate its role in shaping genomic diversity. We used Illumina technology to sequence the
whole genomes of 86 Iberian honeybees, collected across three longitudinal transects in the
Iberian Peninsula and spanning semi-arid climates in the southeastern peninsula to oceanic in
the North-West. The dataset was first analyzed for FST-outliers, CLR (composite-likelihood
ratio) and EHH (Extended Haplotype Homozygosity) methods were further deployed to
evaluate polymorphisms implicated in local adaptation and possibly in the response to human-
mediated environmental changes, including known and novel variants in genes related to
behavior, vision, xenobiotic detoxification and immune response.
Description
Keywords
Pedagogical Context
Citation
Henriques, Dora; Wallberg, Andreas; Rufino, José; Chávez-Galarza, Julio; Costa, Filipe; Webster, Mathew; Pinto, M. Alice (2015). Searching for signatures of selection in Iberian honey bee (Apis mellifera iberiensis) using whole genome sequences. In Congress of the European Society for Evolutionary Biology. Lausanne, Switzerland
Publisher
Universite de Lausanne