Sampaio, Igor Carvalho FontesMoura, Isabela Viana Lopes deCrugeira, Pedro Jorge LouroChinalia, Fábio AlexandreMatos, Josilene Borges Tores LimaJones, Cleveland MaximinoAlmeida, Paulo Fernando de2024-11-182024-11-182024Sampaio, Igor Carvalho Fontes; Moura, Isabela Viana Lopes de; Crugeira, Pedro Jorge Louro; Chinalia, Fábio Alexandre; Matos, Josilene Borges Tores Lima; Jones, Cleveland Maximino; Almeida, Paulo Fernando de (2024). Exploring Innovative Exogenous Green Stimulus Methods for Boosting Bioprocesses: Electric, Magnetic and Ultrasound Stimulation Techniques. In Trends and Innovations in Energetic Sources, Functional Compounds and Biotechnology: Science, Simulation, Experiments. Cham: Springer Nature, p. 497-521. ISBN 978-3-031-46544-4978-3-031-46544-4978-3-031-46545-1http://hdl.handle.net/10198/30622This book chapter provides an overview of the innovative exogenous green stimulus methods of electric, magnetic, and ultrasound stimulation and their appli- cations in boosting bioprocesses. These non-invasive and non-toxic techniques have been shown to enhance the metabolic activity and stress response of various micro- bial systems, including bacteria, yeasts, fungi, and microalgae, leading to increased yields of valuable bioproducts and bioremediation technologies. The chapter begins with an introduction to the concept of these green stimulus and the rationale behind its use in bioprocessing. This is followed by a discussion of the different principles and mechanisms of electric, magnetic, and ultrasound stimulation, including the physical and biological effects that underpin their efficacy. Additionally, the chapter covers the application of these techniques in various bioprocesses, including micro- bial fermentation, biosynthesis of enzymes, and bioconversion of biomass. Examples are provided to illustrate the benefits of green stimulus in enhancing the production of specific bioproducts, such as biofuels, pharmaceuticals, and biopolymers. Finally, we conclude with a critical evaluation of the potential of these techniques in the bioprocessing industry, highlighting the challenges and limitations that need to be addressed to fully harness their potential.engBioprocess enhancementMetabolic modulationBiostimulationMicrobial stimulationbook part10.1007/978-3-031-46545-1_23