Guillier, LaurentGonzales-Barron, UrsulaPouillot, RégisMota, Juliana De OliveiraAllende, AnaKovacevic, JovanaGuldimann, ClaudiaFazil, AamirAl-Qadiri, HamzahDong, QingliHasegawa, AkioCadavez, VascoSanaa, Moez2025-11-142025-11-142025Guillier, Laurent; Gonzales-Barron, Ursula; Pouillot, Régis; Mota, Juliana De Oliveira; Allende, Ana; Kovacevic, Jovana; Guldimann, Claudia; Fazil, Aamir; Al-Qadiri, Hamzah; Dong, Qingli; Hasegawa, Akio; Cadavez, Vasco; Sanaa, Moez (2025). A Quantitative Risk Assessment Model for Listeria monocytogenes in Ready-to-Eat Cantaloupe. International Journal of Food Sciences and Nutrition. ISSN 2304-8158. 14:13, p. 1-342304-8158http://hdl.handle.net/10198/35073This study introduces a farm-to-fork quantitative risk assessment (QRA) model for invasive listeriosis from ready-to-eat diced cantaloupe. The modular model comprises seven stages-preharvest (soil and irrigation contamination), harvest (cross-contamination and survival), pre-processing (brushing), processing (flume tank washing, dicing and equipment cross-contamination), lot testing, cold-chain transport and retail growth, and consumer storage/handling. Each stage employs stochastic functions to simulate microbial prevalence and concentration changes (growth, inactivation, removal, partitioning, cross-contamination) using published data. In a reference scenario-good agricultural practices (soil barriers, no preharvest irrigation), hygienic processing and proper cold storage-the model predicts low lot- and pack-level contamination, with few packs >10 CFU/g and most servings below detection; the mean risk per serving is very low. "What-if" analyses highlight critical control points: the absence of soil barriers with preharvest irrigation can increase the risk by 10,000-fold; flume tank water contamination has a greater impact than harvest-stage cross-contamination; and poor consumer storage can raise the risk by up to 500-fold. This flexible QRA framework enables regulators and industry to evaluate and optimize interventions-from improved agricultural measures to targeted sampling plans and consumer guidance-to mitigate listeriosis risk from RTE diced cantaloupe.engMelonPrimary productionListeriosisExposure assessmentSimulationjournal article10.3390/foods14132212