Živković, JelenaBarreira, João C.M.Stojković, DejanCebovic, TatjanaSantos-Buelga, CelestinoMaksimović, ZoranFerreira, Isabel C.F.R.2014-08-132014-08-132014Živkovic, Jelena; Barreira, João C.M.; Stojković, Dejan; Cebovic, Tatjana; Santos-Buelga, Celestino; Maksimovic, Zoran; Ferreira, Isabel C.F.R. (2014). Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq. Journal of Functional Foods. ISSN 1756-4646. 9:1, p. 192-2011756-4646http://hdl.handle.net/10198/10144This study was designed to characterize the phenolic profile and evaluate the antibacterial, antimutagenic and antitumor activities of Veronica urticifolia Jacq. methanolic extract. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumor activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumor cell viability, while ascites volume and tumor cell count became slightly decreased, but not to a statistically significant extent. Above results indicate that V. urticifolia deserves further research into chemoprevention.engVeronica urticifoliaPhenolic profileHPLC-DAD/ESI-MSAntibacterialAntimutagenicityEhrlich carcinomajournal article10.1016/j.jff.2014.04.024