Meirinho, Sofia G.Dias, L.G.Peres, António M.Rodrigues, Lígia R.2018-02-202018-02-202014Meirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2014). Development of an Electrochemical Aptasensor for the Detection of Human Osteopontin. In Eurosensors 2014 - The 28th European Conference on Solid-State Transducres. Brecia, Itáliahttp://hdl.handle.net/10198/15823Electrochemical RNA aptasensor To obtain bio-sensor with high affinity for rhOPN aiming to be used for cancer prognosis Materials and methods RNA aptamer details Isolated by SELEX process [1] Sequence of the biotinylated RNA aptamer 5’-Biotin- CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG-3’ Removal the interference of Rnase Working RNA aptamer solution 4 nM in PBS. RNA aptamer immobilization on a gold surfasse via streptavidin-biotin interactionengconference poster