Fernandes, Filipa AlexandraOliveira, Paula A.Pinto, M.L.Venâncio, CarlosFélix, LuísMedeiros-Fonseca, BeatrizCorreia, RossanaPrieto Lage, Miguel A.Ferreira, Isabel C.F.R.Barros, LillianHeleno, Sandrina A.2024-12-202024-12-202024Fernandes, Filipa A.; Oliveira, Paula A.; Pinto, Maria L.; Venâncio, Carlos; Félix, Luis; Medeiros-Fonseca, Beatriz; Correia, Rossana; Prieto, Miguel A.; Ferreira, Isabel C.F.R.; Barros, Lillian; Heleno, Sandrina A. (2024). Effects of a Cyperus esculentus L. plant-based beverage on FVB/n female mice. Food and Chemical Toxicology. ISSN 0278-6915. 194, p. 1-100278-6915http://hdl.handle.net/10198/30770This work evaluated the effects of the consumption of a plant-based beverage (Cyperus esculentus L., Adansonia digitata L., and thermal water) on the physiological parameters of mice over a 28-day period. Thirty-two female FVB/n mice (n = 8) were randomly assigned to one of four groups divided into two experimental protocols: Group 1 drank water and Group 2 drank a plant-based beverage for 24 h; in the second experimental protocol, Group 3 consumed water and Group 4 consumed plant-based beverage for a limited time (4 h). Two experimental protocols were conducted to assess whether the exposure time to the beverage affects the animals’ physiological parameters, to determine if there is a possible daily limit for consumption of the product and to analyze their adverse effects. The mice consuming a beverage ingested a larger amount of drink and a smaller amount of food. Histologically there are no pathological changes in collected organs. The consumption of a plant-based beverage has been shown to have a positive effect on oxidative markers and can have a diuretic action. According to results, no behavioral changes or clinical signs of disease were observed throughout both experimental protocols, and no mortality was recorded.engPlant-based beverageCyperus esculentus L.In vivo safety assessmentFVB/Njournal article10.1016/j.fct.2024.115082