Ziani, Borhane E.C.Carocho, MárcioAbreu, Rui M.V.Bachari, KhaldounAlves, Maria JoséCalhelha, Ricardo C.Talhi, OualidBarros, LillianFerreira, Isabel C.F.R.2018-02-192020-06-222018-01-192020-06-222020Ziani, Borhane E.C.; Carocho, Marcio; Abreu, Rui M.V.; Bachari, Khaldoun; Alves, Maria José; Calhelha, Ricardo C.; Talhi, Oualid; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Phenolic profiling, biological activities and in silico studies of Acacia tortilis (Forssk.) Hayne ssp. raddiana extracts. Food Bioscience. ISSN 2212-4292. 36, p. 1-102212-4292http://hdl.handle.net/10198/16706To evaluate the phenolic profile and biological activity of the Algerian Sahara plant Acacia tortilis (Forssk.) Hayne ssp. raddiana decoction and 80% ethanolic extracts were studied. Chromatographic profiling indicated the presence of 36 phenolic compounds, including gallic acid esterified derivatives, galloylquinic derivatives and flavan-3-ols galloyl derivatives. Both extracts showed significant cytotoxic activity and a potent anti-inflammatory activity. They were effective against several multi-drug resistant bacteria, namely methicillin-resistant Staphylococcus aureus (MRSA). To understand the possible mechanism of action of MRSA inhibition activity, an in silico docking analysis was done using a virtual library of the 36 determined phenolic compounds against penicillin-binding protein (PBP2a), a protein known to be involved in MRSA resistance to β-lactam antibiotics. A. tortilis extracts showed interesting biological activities and the phenolic compounds found could be an interesting starting point for the development of cytotoxic and anti-inflammatory drugs and especially anti-MRSA antibiotics.engAcacia tortilisAnti-inflammatoryAntimicrobialCytotoxicityMolecular dockingPhenolic compoundsjournal article10.1016/j.fbio.2020.100616