Taboada, Bruna Rafaela PereiraMonteiro, Fernando C.Lima, Rui A.2018-04-062018-04-062016Taboada, B.; Monteiro, Fernando C.; Lima, R. (2016)- Automatic tracking and deformation measurements of red blood cells flowing through a microchannel with a microstenosis: the keyhole model. Computer Methods in Biomechanics and Biomedical Engineering: Imaging and Visualization. ISSN 2168-1163. 4:3-4, p. 229-2372168-1163http://hdl.handle.net/10198/16722This study aimed to assess the motion and its deformation index (DI) of red blood cells (RBCs) flowing through a microchannel with a microstenosis using an image analysis-based method. For this purpose, a microchannel having a smooth contraction was used and the images were captured by a standard high-speed microscopy system. An automatic image-processing and analysing method was developed in a MATLAB environment to not only track the motion of RBCs but also measure the DI along the microchannel. The keyhole model, tested in this study, proved to be a promising technique to automatically track individual RBCs in microchannels.engAutomatic trackingDeformation indexKeyhole modelMicrofluidsRed blood cellsjournal article10.1080/21681163.2014.957868