Li, FernandaRosa-Fontana, AnneliseYadró Garcia, Carlos A.Rufino, JoséVerbinnen, GillesGraaf, Dirk C.Smet, Lina dePinto, M. AliceHenriques, Dora2024-11-052024-11-052024Li, Fernanda; Rosa-Fontana, Annelise; Yadró Garcia, Carlos Ariel; Rufino, José; Verbinnen, Gilles; de Graaf, Dirk C.; Smet, Lina de; Pinto, M. Alice; Henriques, Dora (2024). Diversity patterns of P450 and ABC transporter genes in 17 honey bee subspecies. In 16th International Symposium Hazards of Pesticides to Bees. Sevilla, p. 70-71http://hdl.handle.net/10198/30501Honey bees (Apis mellifera) inhabit a vast geographical range, spanning diverse natural and agricultural ecosystems. They are exposed to different levels and types of natural (such as plant allelochemicals) and synthetic (such as pesticides) xenobiotics within this range. Several genes have been implicated in the resistance of insects to pesticides, including the P450 monooxygenases superfamily and ATP-binding cassette sub-family F member 1that contain 46 and 41 genes, respectively. Here, the sequences of P450 monooxygenases and ABC transporters from >1500 individuals representing 17 subspecies of the four honey bee main lineages will be analyzed. The functional annotation and effects of each variant will then be predicted using SnpEff and the allele frequency and FST (fixation index) of each SNP per population and evolutionary lineages will be calculated. It is expected to have highly differentiated SNPs among the different subspecies/lineages.engP450ABC transporterDiversityHHoney beesconference object