Silva, Gabriela Catuzo CanonicoMachado, Mariane de AlmeidaSakumoto, KarinaInumaro, Rodrigo SadaoGonçalves, José EduardoMandim, FilipaVaz, Josiana A.Valle, Juliana Silveira doFaria, Maria Graciela IecherRuiz, Suelen PereiraPiau Junior, RanulfoGonçalves, Daniela DibGazim, Zilda Cristiani2012-03-092012-03-092023Silva, Gabriela Catuzo Canonico; Machado, Mariane de Almeida; Sakumoto, Karina; Inumaro, Rodrigo Sadao; Gonçalves, José Eduardo; Mandim, Filipa; Vaz, Josiana A.; Valle, Juliana Silveira do; Faria, Maria Graciela Iecher; Ruiz, Suelen Pereira; Piau Junior, Ranulfo; Gonçalves, Daniela Dib; Gazim, Zilda Cristiani (2023). Cellular antioxidant, anti-inflammatory, and antiproliferative activities from the flowers, leaves and fruits of Gallesia integrifolia spreng harms. Molecules. eISSN 1420-3049. 28:14, p. 1-24http://hdl.handle.net/10198/6684Gallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC-MS and CEs by UHPLC-MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2 & PRIME;-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI(50) between 51 and 230 & mu;g/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 & mu;g/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.engjournal article10.3390/molecules281454061420-3049