Pinho, DianaLima, Rui A.Pereira, Ana I.Gayubo, Fernando2014-09-082014-09-082013Pinho, D.; Lima, R.; Pereira, Ana I.; Gayubo, F. (2013). Automatic tracking of labeled red blood cells in microchannels. International Journal for Numerical Methods in Biomedical Engineering. ISSN 2040-7947. 29, p. 977-987http://hdl.handle.net/10198/10355The current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- m glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland–Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels.engImage analysisAutomatic methodRed blood cellsIn vitro blood flowjournal article10.1002/cnm.2526