Pires, Tânia C.S.P.Dias, Maria InêsBarros, LillianCalhelha, Ricardo C.Oliveira, BeatrizSantos-Buelga, CelestinoFerreira, Isabel C.F.R.2018-02-122018-02-122017Pires, Tânia C.S.; Dias, Maria Inês; Barros, Lillian; Calhelha, Ricardo; Oliveira, M.B.P.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2017). Rosa canina L. as new food ingredient an source of bioactive compounds. In 3rd Symposium on Medicinal Chemistry of University of Minho. Bragahttp://hdl.handle.net/10198/15685Sustainable food options are increasing among the most concerned consumers, in which they seek to combine new ingredients with potential health benefits [1]. Edible flowers provide new colors, textures and vibrancy to any dish, and apart from the "glam" factor, they can provide bioactive compounds [2]. In the presentwork, the edible petals of Rosa canina L, gently supplied by the RBR Foods Company (Portugal), were tested for their cytotoxic activity against tumor cell lines. The assays were conducted in the hydromethanolic extract and in the lyophilized infusion, being both of them re-dissolved in water in order to obtain stock solutions at 100 mg/mL for successive dilutions until determination of Glso values (concentration that inhibits 50% of the net cell growth). Four human tumor cell lines were tested: MCF-7 (breast adenocarcinoma), NCIH460 (non-small cell lung câncer), HeLa (cervical carcinoma) and HepG2 (hepatocellular carcinoma). A non-tumour primary cells' culture (assigned as PLP2) was also prepared from a freshly harvested porcine liver, and used in the bioassays. Ellipticine was used as the positive contrai. While the hydromethanolic extract inhibited the growth of HeLa (308 pg/mL), the lyophilized infusion inhibited MCF-7 (377 pg/mL), respectively. Both extracts presented cytotoxicity for HepG2 (297 |jg/mL and 315 pg/mL for hydromethanolic extract and infusion, respectively). Nane of the extracts showed toxicity towards PLP2. After chemical characterization of the extracts, flavonoids were the only phenolic compounds present, being quercetin-3-O-glucoside and kaempferol-3-O-glucoside the major compounds present in both extracts. These results showed that R. canina edible flowers are sources of bioactive compounds related with cytotoxic properties in human tumor cell lines, highlighting their applicability potential in nutraceutical and pharmaceutical fields.engconference object