Meirinho, Sofia G.Ezziddine, MahaVeloso, Ana C.A.Dias, L.G.Rodrigues, Lígia R.Othmane, AliPeres, António M.2020-02-272020-02-272017Meirinho, Sofia G.; Ezzedine, Maha; Veloso, Ana C.A.; Dias, L.G.; Rodrigues, Lígia R.; Othmane, Ali; Peres, António M. (2017). Optimization of the aptamers’ immobilization conditions for maximizing the response of a dualaptasensor for cancer biomarker detection. In XIV Reunión Ibernam. Tordesillashttp://hdl.handle.net/10198/20731A simple and sensitive method that allows the simultaneous detection of single or multiple cancer biomarkers is envisaged and may be an important tool in cancer diagnosis. Osteopontin (OPN) is a protein that is present in several body fluids and has been reported as a possible cancer biomarker. The voltammetric signals generated by the dual-aptasensor array, after the formation of the aptamers-protein complex, were monitored using cyclic voltammetry (CV) and square-wave voltammetry (SWV), using [Fe(CN)6] 3/ 4 as a redox probe. The aim of this work is to optimize the experimental conditions to development a label-free voltammetric dual-aptasensor array for the detection of human osteopontin using a 2k factorial experimental design.engconference poster