Mota, PaulaSoares, João F.Moreira, Maria Helena RodriguesLeitão, José C.Freitas, Joana Isabel MartinsMonteiro, A.M.Figueiredo, PedroDuarte, José Alberto2012-09-172012-09-172008Mota, M.P.; Soares, J; Moreira, M.H.; Leitão, J.C.; Freitas, Joana Isabel Martins; Monteiro, A.M.; Figueiredo, P.; Duarte, J.A. (2008). Influence of hormonal replacement therapy in lipid peroxidation levels of postmenopausal women with different cardiovascular capacity. In 12th World Congress on the Menopause e Climacteric: The Journal Of The International Menopause Society. ISSN 1473-0804. Vol. 11, Suppl 2, p. 2041473-0804http://hdl.handle.net/10198/7573It has been suggested that exercise has a positive impact on the prevention and progression of cardiovascular disease (CVD). One of the main mechanisms is through the modification of lipoprotein levels and the risk of its oxidation, especially LDL lipoproteins. After menopause, women experience an increased incidence of cardiovascular disease. In contrast, women receiving hormonal replacement therapy (HRT) seem to be protected. The aim of this study was to infer how HRT affect lipid peroxidation levels in postmenopausal women with different levels of aerobic fitness. Design & Method: Sixty four women participate in this study, 32 of them receive HRT (group with HRT – GWHRT) (average age¼55.9 years, average weight¼ 66.9 kg and average high¼156.0 cm) and the remained 32 women didn’t receive HRT (group without HRT – GWOHRT) (average age¼61.0 years, average weight¼ 67.6 kg and average high¼154.3 cm). Total cholesterol (mg dL71), HDL-cholesterol (mg dL71), LDL-cholesterol (mg dL71) and triglycerides levels (mg dL71) have been analyzed in serum obtained from a blood sample collected after 8 hours of fasting, and measured in Dr. Lange LP20 according to the specific manufacturer instructions. Serum MDA concentration (ng L71) was determined by spectrophotometric method. Aerobic capacity (VO2max ml kg71 min71) was assessed according to an adaptation of Bruce protocol. Differences between groups were tested through Student t-test. A Spearman correlation was performed in order to test variables associations. Significance level was established at 5%.Results: Our results have found differences between groups in age (t¼3.018; p50.01), in VO2max (t¼73.774; p50.01) and in serum MDA concentration (t¼6.750; p50.01). The GWHRT were younger, had a higher VO2max (31.38 for GWHRT and 26.19 for GWOHRT), and had lower levels of serum MDA concentration (0.29 for GWHRT and 0.73 for GWOHRT) comparatively with those women from the GWOHRT. However, our results failed to find any differences between groups regarding serum triglycerides, total cholesterol, HDL-cholesterol, LDL-cholesterol or BMI. In this study, serum MDA concentration didn’t correlate with higher levels of lipid profile, as we might have expected, but correlate inversely with HRT (r¼7.726, p¼.00) and with VO2max (r¼7.287, p¼.02). Conclusion: Our results suggest that HRT should exert an antioxidant protective effect against lipid peroxidation, as well as exercise perhaps through the stimulation of antioxidant capacity.engconference object