Barros, LillianCorreia, Daniela M.Ferreira, Isabel C.F.R.Baptista, PaulaSantos-Buelga, Celestino2008-09-122008-09-122008Food Chemistry. ISSN 0308-8146. 110:4 (2008) p. 1046-10500308-8146http://hdl.handle.net/10198/838Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid–liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.engVitamin EHPLC AnalysisMushroomsjournal article10.1016/j.foodchem.2008.03.016