Ziani, Borhane E.C.Heleno, Sandrina A.Bachari, KhaldounDias, Maria InêsAlves, Maria JoséBarros, LillianFerreira, Isabel C.F.R.2018-02-192019-01-282018-01-192019-01-282019Ziani, Borhane E.C.; Heleno, Sandrina A.; Bachari, Khaldoun; Dias, Maria Inês; Alves, Maria José; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Phenolic compounds characterization by LC-DAD- ESI/MSn and bioactive properties of Thymus algeriensis Boiss. & Reut. and Ephedra alata Decne. Food Research International. ISSN 0963-9969. 116, p. 312-3190963-9969http://hdl.handle.net/10198/18569Scientific research has been focused on finding natural occurring molecules from plant origin. Herein, infusion, decoction and hydroethanolic extracts of Thymus algeriensis Boiss. & Reut. and Ephedra alata Decne. from Algeria were phytochemically characterized by LC-DAD-ESI-MSn, and evaluated regarding bioactive properties (antioxidant and antibacterial). Flavonol and flavone glycoside derivatives and phenolic acids, specially rosmarinic acid and kaempferol-O-glucuronide were the major compounds in T. algeriensis extracts. Otherwise, E. alata presented isoflavones and flavonol derivatives as main compounds, being hydroxypuerarin isomer 1 the major molecule. Aqueous extracts had significantly higher antioxidant activity, being this activity correlated with the amount of phenolic compounds. Antimicrobial activity of the extracts was tested against multi-resistant bacteria strains from clinical isolates. The obtained MIC values indicate that the hydroethanolic extracts revealed the highest effect, especially the one of T. algeriensis against Gram-positive bacteria. Hence, these plant extracts could be used in the development of bioactive ingredients.engEphedra alataInfusion/decoction/hydroethanolic extractPhenolic profile/bioactivitiesThymus algeriensisjournal article10.1016/j.foodres.2018.08.041