Abir, TebaiOliveira, IzamaraFalcão, SoraiaBen Rejeb, InesBarros, LillianHeleno, Sandrina A.2023-03-162023-03-162022Abir, Tebai; Oliveira, Izamara; Falcão, Soraia; Rejeb, Ines Bem; Barros, Lillian; Heleno, Sandrina A. (2022). Antimicrobial activity of rosemary, eucalyptus and propolis individual and mixed extracts. In XXVI Encontro Galego-Portugues de Química: Book of Abstracts. Santiago de Compostela978-84-09-45895-0http://hdl.handle.net/10198/27797Preservative-free meals and the use of natural products as natural alternatives are becoming more popular as consumers become more aware of the potential health problems associated with artificial food preservatives. The current study focused on exploring the potential of plant extracts (rosemary and eucalyptus) and propolis, or their mixtures, to develop a highly bioactive formulation. Extracts were prepared individually and mixtures of eucalyptus/propolis, eucalyptus/rosemary, and rosemary/propolis using hydroethanolic extracts (80% ethanol) in different ratios of 50:50, 60:40, and 40:60, respectively., were also prepared. The selected matrices were prepared by two different maceration processes: i) 1 g of the sample was macerated in 30 mL of 80% hydroethanolic solution using magnetic stirring; ii) 1 g of sample in 10 mL of hydroethanolic solution was macerated in a water bath at 70°C, this being, the recommended protocol for extraction of phenolic compounds from propolis [1]. The extracts were further analyzed for their antimicrobial ability by microdilution method using pathogenic microorganisms. The individual extracts of rosemary and eucalyptus showed remarkably strong antibacterial activity against most bacterial strains, often equivalent to that obtained with the common antibiotic’s streptomycin, methicillin, and ampicillin. Both mixtures, eucalyptus/rosemary (60:40) collected from maceration by magnetic stirring and eucalyptus/propolis (60:40) derived from maceration with water bath, showed the most promising results, with inhibition ability against all bacteria. As for the antifungal ability, no inhibition was obtained at the maximum tested concentration of 10 mg/mL. In addition to these encouraging results for antimicrobial activity, further bioactivity assays will be performed to evaluate the properties of the different extracts.engRosemaryEucalyptusPropolisResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologyconference object