Leblebici, PelinRodrigues, AlírioPais, Luís S.2014-01-152014-01-152013Leblebici, P.; Rodrigues, A.E.; Pais, L.S. (2013). Separation of human immunoglobulin G subclasses using monolithic column. In 9th European Congress of Chemical Engineering and 2nd European Congress of Applied Biotechnology. Hague, Netherlandshttp://hdl.handle.net/10198/9152The present study aims to separate hIgG into its subclasses using monolithic stationary phase.Human immunoglobulin G (hIgG), constitutes an important therapeutic antibody for a number of diseases. (1) There is an increasing demand for highly purified hIgG and its sublasses since wide range of potential application in immunodiagnostics and immunotherapy was found. (2) hIgG consists of four subclasses (IgGI, IgG2, IgG3, and IgG4) that show differences in some of their physicochemical characteristics and biological properties.engHuman immunoglobulin GMonolithic stationary phaseAffinity chromatographyProtein Aconference object