Maleita, Carla Maria NobreCosta, Sofia R.Abrantes, Isabel2018-02-192019-01-282018-01-192019-01-282018Maleita, Carla Maria Nobre; Costa, Sofia R.; Abrantes, Isabel (2018). Laimaphelenchus suberensis sp. nov. associated with Quercus suber in Portugal. European Journal of Plant Pathology. ISSN 0929-1873. 150, p. 747-7580929-1873http://hdl.handle.net/10198/18550Laimaphelenchus suberensis sp. nov. obtained from declining Quercus suber trees of Herdade da Gouveia de Baixo, Alentejo, Portugal, is described and illustrated based on morphological, biometrical and molecular characters. The diagnosis of Laimaphelenchus species has been commonly based on the presence or absence of a vulval flap and on the shape structure of the tail tip. The species described here has been included in the Laimaphelenchus group without vulval flap, and can be distinguished from morphologically similar species by its tail tip shape structure that has a stalk-like terminus and three diffuse tubercles with 4–6 finger-like protrusions. For the molecular analyses, the mitochondrial DNA region from the cytochrome oxidase subunit I (mtCOI), the D2-D3 expansion segments of the large subunit (LSU) and small subunit (SSU) of rRNA gene were amplified and sequenced. Sequences of L. suberensis sp. nov. clustered separately from all Laimaphelenchus spp. with available sequences in Genbank, confirming its identification as a new species. This is the second report of the genus Laimaphelenchus in Portugal, associated with Q. suber: L. heidelbergi and L. suberensis sp. nov.engCork oakCytochrome oxidase subunit ILarge subunit ribosomal RNAMolecular analysisMorphologySmall subunit ribosomal RNAjournal article10.1007/s10658-017-1324-4