Lageiro, ManuelaCastanho, AnaPereira, CristianaCalhelha, Ricardo C.Ferreira, Isabel C.F.R.Brites, Carla2018-02-192021-02-272018-01-192021-02-272020Lageiro, M. Manuela; Castanho, Ana; Pereira, Cristiana; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Brites, Carla (2020). Assessment of gamma oryzanol variability, an attractive rice bran bioactive compound. Emirates Journal of Food and Agriculture. ISSN 2079-052X. 32:1, p. 38-462079052Xhttp://hdl.handle.net/10198/23428Gamma-oryzanol is a compound present on rice bran, which has been studied for its bioactive properties, including its cytotoxic activity. The gamma-oryzanol biosynthesis occurs on the lipidic membrane of the plant and can be influenced by edaphoclimatic grown conditions. Two Portuguese rice varieties (Ceres and Macarico) from 6 growing environments were analysed in order to explore the influence of the growing conditions on the gamma-oryzanol content, its constituents, and cytotoxic effect against 4 human tumour cell lines (NCI-H460, HeLa, HepG2, and MCF-7). The overall variability of gamma-oryzanol concentration was from 1.56 g/Kg to 3.19 g/Kg, Ceres reveals higher values than Macarico; those concentrations also varied with the growing environment. The environment was also determinant for the individual concentration of the 3 gamma-oryzanol compounds (cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate). A cytotoxic effect was observed for all the tested tumour cell lines and also varied with the growing environment.engCytotoxicityGamma-oryzanolHPLCRice branjournal article10.9755/EJFA.2020.V32.I1.2056