Pereira, Olívia R.Perez, María J.Macias, Rócio I.R.Domingues, Maria R.M.Silva, ArturMarín, Jose J.G.Cardoso, Susana M.2013-03-122013-03-122012Pereira, Olívia R.; Perez, Maria J.; Macias, Rocio I. R.; Domingues, Maria R. M.; Silva, Artur M. S.; Marin, Jose J. G.; Cardoso, Susana M. (2012). Cytisus multiflorus: source of antioxidant polyphenols. In 11º Encontro Química dos Alimentos. Bragança978-972-745-132-6http://hdl.handle.net/10198/8211The present study investigates the phenolic composition and the antioxidant capacity of an ethanolic extract from flowers of C. multiflorus. As determined by HPLC-DAD, ESI-MSn and NMR combined analysis, this extract was mainly composed of chrysin-7-O-β-D-glycopyranoside, and also contained considerable amounts of rutin, a dihydroxyflavone isomer of chrysin and glycosidic derivatives of luteolin and apigenin. The C. multiflorus ethanolic extract exhibited high DPPH scavenging activity and reducing power with EC50 of 13.4 ± 0.6 and 95.7 ± 4.6 µg/mL, respectively. The exposure of human hepatoblastoma HepG2 cells to the non-toxic concentrations of the extract (50 or 200 µg/mL) resulted in a decreased rate of ROS production in a concentration dependent manner. Moreover, the mixture of standards that simulated the phenolic composition of the plant afforded a protection of about 50% in intracellular ROS production. Hence, the gathered results suggest that C. multiflorus polyphenols are closely associated to its antioxidant properties.engCytisus multiflorusPhenolic compoundsAntioxidant activityconference object