Barreira, João C.M.Ferreira, Isabel C.F.R.Oliveira, BeatrizPereira, J.A.2011-03-232011-03-232007Barreira, João C.M.; Ferreira, Isabel C.F.R.; Oliveira, M. Beatriz P.P.; Pereira, José Alberto (2007). Antioxidant activity of chestnut constituents: flowers, leaves, skins and fruits. In II Congresso Ibérico do Castanheiro. Vila Realhttp://hdl.handle.net/10198/3731ln this study, the antioxidant properties 01 different chestnut constituents (1Iowers, leaves, skins and fruits) were evaluated through several biochemical assays: DPPH (2 ,2- diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition 01 ~carotene bleaching, inhibition 01 oxidative hemolysis in erythrocytes, induced by 2,2'azobis( 2-amidinopropane)dihydrochloride (AAPH), and inhibition 01 lipid peroxidation in pig brain tissue through formation 01 thiobarbituric acid reactive substances (TBARS). These assays have been extensively studied as models for the peroxidative damage in biomembranes. For all the methods EC50 values were calculated in order to evaluate the antioxidant efficiency 01 each product. The phenol and flavonoid contents were also obtained and correlated to antioxidant activity. Chestnut skins revealed much better antioxidant properties, presenting much lower EC50 values, particularly lor lipid peroxidation inhibition in TBAR8 assay. Also, the highest antioxidant contents (phenols and flavonoids) were found for this constituent.engAntioxidant activity of chestnut constituents: flowers, leaves, skins and fruitsconference object