Bento, DavidPinho, DianaPereira, Ana I.Lima, Rui A.2014-10-302014-10-302011Bento, David; Pinho, Diana; Pereira, Ana I.; Lima, R. (2012) . Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method. In International Conference on Numerical Analysis and Applied Mathematics. Greece. p.786-789http://hdl.handle.net/10198/11216In this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i. e., a manual method (MM) and an automatic method (AM). For theMM we have used amanual tracking plugin (MTrackJ) from Image J to track labeled red blood cells (RBCs) flowing around the boundary of the RBCs core. For the AM we have used a MatLab scripts to measure automatically the CFL trajectories. The preliminary numerical results suggest that the CFL trajectories follow a polynomial function for both methods.engCell-free layerAutomatic methodRed blood cellsNonlinear optimizationconference object