Kowalczyk, AlicjaKowalewska-Łuczak, IngaCzerniawska-Piątkowska, EwaCantalapiedra, Jesús J.Cerqueira, J.O.L.Araújo, José Pedro2018-02-192018-08-032018-01-192018-08-032023Kowalczyk, Alicja; Kowalewska-Łuczak, Inga; Czerniawska-Piątkowska, Ewa; Cantalapiedra, Jesús Juan; Cerqueira, J.O.L.;Araújo, José Pedro. (2023). Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls. Zoological Society of Pakistan. ISSN 00309923. 55:1, p. 1-60030-9923http://hdl.handle.net/10198/17332The purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.engAQP7 geneHolstein-Friesian bullsAQP7 polymorphismReproductionSperm cells qualityAquaporinsAquaglycero-porinsSingle nucleotide polymorphismsjournal article10.17582/journal.pjz/20210901170933