Areias, Filipe M.Valentão, PatríciaAndrade, Paula B.Moreira, Manuela M.Amaral, Joana S.Seabra, Rosa M.2010-02-082010-02-082000Areias, F.M.; Valentão, P.; Andrade, P.B.; Moreira, M.M.; Amaral, J.S.; Seabra, R.M. (2000). HPLC/DAD analysis of phenolic compounds from lavender and its application to quality control. Journal of Liquid Chromatography & Related Technologies. ISSN 1520-572X. 23(16), p.2563–25721520-572Xhttp://hdl.handle.net/10198/1754A reversed phase HPLC procedure is proposed for the determination of eight phenolic compounds (2-O-glucosilcoumaric acid, o-coumaric acid, rosmarinic acid, apigenin-7-O-glucoside, coumarin, herniarin, luteolin, and apigenin) in lavender. The chromatographic separation was achieved using a reversed–phase Spherisorb ODS 2 (5 μm particle size: 25.0 x 0.46 cm) column. From the several extractive solvents assayed, only ethanol was able to extract all the mentioned compounds. Best resolution was obtained using a gradient of water-formic acid (19:1) and acetonitrile. Ten samples were subjected to quantification, all of them showing a common composition pattern.engjournal article10.1081/JLC-100100510