Utilize este identificador para referenciar este registo: http://hdl.handle.net/10198/9307
Título: A SYBR green real-time PCR assay to detect and quantify pork meat in processed poultry meat products
Autor: Soares, S.
Amaral, J.S.
Oliveira, M.B.P.P.
Mafra, I.
Palavras-chave: Quantitative real-tome PCR
SYBR Green
Data: 2013
Editora: Elsevier
Citação: Soares, S.; Amaral, J.S.; Oliveira, M.B.P.P.; Mafra, I. (2013) - A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products. Meat Science. ISSN 0309-1740. 94, p.115-120
Resumo: Species identification in meat products has grown in interest in recent years since these foodstuffs are susceptible targets for fraudulent labelling. In this work, a real-time PCR approach based on SYBR Green dye was proposed for the quantitative detection of pork meat in processed meat products. For the development of the method, binary meat mixtures containing known amounts of pork meat in poultry meat were used to obtain a normalised calibration model from 0.1 to 25% with high linear correlation and PCR efficiency. The method revealed high specificity by melting curve analysis, being successfully validated through its application to blind meat mixtures, which confirmed its adequacy for pork meat determination. The fully applicability of the method was further demonstrated in commercial meat products, allowing verification of labelling compliance and identification of meat species in processed foods.
Peer review: yes
URI: http://hdl.handle.net/10198/9307
Aparece nas colecções:DTQB - Artigos em Revistas Indexados ao ISI/Scopus

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