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|Título: ||Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources|
|Autor: ||Barros, Lillian|
Estevinho, Leticia M.
Ferreira, Isabel C.F.R.
|Palavras-chave: ||Mushroom mycelium|
|Issue Date: ||2007|
|Citação: ||Barros, Lillian; Baptista, Paula; Estevinho, Letícia M.; Ferreira, Isabel C.F.R. (2007) - Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources. Food Chemistry. ISSN 0308-8146. 105:1, p. 179-186|
|Resumo: ||Leucopaxillus mushroom species are used in the chemical industry for extraction of clitocybin antibiotic and particularly the edible mushroom Leucopaxillus giganteus can be found in Northeast Portugal. Nevertheless, the production of its mycelium for pharmacological
applications has not been explored. Herein, the mycelium obtained in the presence of four different nitrogen sources was investigated
with regard to phenol production and antimicrobial and antioxidant properties. Phenol concentration increased along the growth time as
a response to the oxidative stress and therefore free radical production. Although significant differences for mycelium growth between the
nitrogen sources had not been observed, (NH4)2HPO4 proved to be the most appropriate to increase bioactive properties, leading to the
highest phenol content and lowest EC50 and MIC values. The antimicrobial capacity was screened against Gram positive and Gram negative
bacteria, and fungi. The samples selectively inhibited the growth of Gram positive bacteria, Staphylococcus aureus being the most
susceptible one. Gram negative bacteria and fungi were resistant to the extracts. Antioxidant activity was calculated by the reducing
power assay, the scavenging effect on DPPH radicals, and hemolysis and lipid peroxidation inhibition. Significantly negative linear
regressions were established between phenols/flavonoids contents, which increased along the mycelia growth time and antioxidant
|Appears in Collections:||BB - Artigos em Revistas Indexados ao ISI|
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