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Título: Antioxidants in Pinus Pinaster and mycorrhizal fungi during the early steps of symbiosis
Autor: Carocho, Márcio
Barros, Lillian
Barreira, João C.M.
Ferreira, Isabel C.F.R.
Martins, Anabela
Issue Date: 2012
Citação: Carocho, Márcio; Barros, Lillian; Barreira, João C.M.; Ferreira, Isabel C.F.R.; Martins, Anabela (2012) - Antioxidants in Pinus Pinaster and mycorrhizal fungi during the early steps of symbiosis. In 7º Encontro Nacional de Cromatografia. Porto
Resumo: Ectomycorrhizal symbiosis between fungi and the roots of some plants could have important effects in t he levels of antioxidants of both partners [1,2]. In the present work, the effects of eo-culture period (6, 24 and 72 h) in the antioxidant properties and antioxidants production duri ng early steps of in vitro mycorrhization (Pinus pinaster - Pisolithus arhizus and Pinus pinaster - Paxillus involutus) were evaluated. The studied parameters were determined in each culture element (root, mycelium and medium) in order to understand the response of each part ner to the symbiot ic association. The antioxidant properties were determined by assays evaluating free radicals scavenging activi ty, reducing power, ~-ca rotene bleaching inhibition and lipid peroxidation inhibition in animal brain homogenates. The concent rations in phenolics, tocopherols and suga rs were obtained by Folin Ciocalteus assay, HPLC-fluorescence and HPLC-RI, respectively. P. arhizus proved to be more compatible with P. pinaster than P. involutus, since the antioxidant activity in the latter species increased from 48 to 72 h, while P. arhizus antioxidant properties decreased at the same period (less oxidative stress). Despite P. involutus proved to be less suitable to be included in forestation programs using mycorrhization processes, it revealed a higher potential for bioactive compounds production in the early steps of symbiosis. A maximal phenolics content was obtained after the first 6 h and, considering bioactive compounds production purposes, the eoculture should be stopped at t hat time. Tocopherols (except y-tocopherol) contents showed high similarities among culture medium and mycelia. This tendency could not be observed regarding sugars contents, which might indicate that the differences in t he chemical responses that characterize each mycorrhization might be related more directly with sugar (primary metabolites) exchanges.
Arbitragem científica: yes
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