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Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/723

Título: Development and evaluation of an HPLC/DAD method for the analysis of phenolic compounds from olive fruits
Autor: Vinha, A.F.
Silva, B.M.
Andrade, P.B.
Seabra, R.M.
Pereira, J.A.
Oliveira, M.B.P.P.
Palavras-chave: High performance liquid chromatography
Diodes
Phenols
Issue Date: 2002
Editora: Marcel Dekker
Citação: Journal Liquid Chromatography & Related Technologies. ISSN 1082-6076. 25:1 (2002) p. 51-160.
Resumo: A new HPLC/DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobrançosa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step. The chromatographic separation was achieved using a Spherisorb ODS2 (25.0 × 0.46 cm; 5 μm, particle size) column. The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL/min. The detection limit values for phenolic compounds were between 0.04 and 4.32 μg/mL and the method was precise. As a general rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivars
URI: http://hdl.handle.net/10198/723
ISSN: 1082-6076
Appears in Collections:CIMO - Artigos em Revistas Indexados ao ISI
PTV - Artigos em Revistas Indexados ao ISI

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Vinha et al., 2002, J. Liq. Chrom. & Rel. Technolo., 25, 151-160.pdf1,15 MBAdobe PDFView/Open

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