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Title: Development and evaluation of an HPLC/DAD method for the analysis of phenolic compounds from olive fruits
Authors: Vinha, A.F.
Silva, B.M.
Andrade, P.B.
Seabra, R.M.
Pereira, J.A.
Oliveira, M.B.P.P.
Keywords: High performance liquid chromatography
Issue Date: 2002
Publisher: Marcel Dekker
Citation: Vinha, A.F.; Silva, B.M.; Andrade, P.B.; Seabra, R.M.; Pereira, J.A.; Oliveira, M.B.P.P. (2002) - Development and evaluation of an HPLC/DAD method for the analysis of phenolic compounds from olive fruits. Journal Liquid Chromatography & Related Technologies. ISSN 1082-6076. 25:1, p. 51-160
Abstract: A new HPLC/DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobrançosa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step. The chromatographic separation was achieved using a Spherisorb ODS2 (25.0 × 0.46 cm; 5 μm, particle size) column. The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL/min. The detection limit values for phenolic compounds were between 0.04 and 4.32 μg/mL and the method was precise. As a general rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivars
Peer review: yes
ISSN: 1082-6076
Appears in Collections:PTV - Artigos em Revistas Indexados ao ISI/Scopus

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