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Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/7011

Título: Size fractionation by slalom chromatography and hydrodynamic chromatography
Autor: Dias, Ricardo P.
Palavras-chave: Separation by flow
Hydrodynamic chromatography
Slalom chromatography
Particle size distribution
Synthetic polymers
Biopolymers
Starch
DNA
Issue Date: 2008
Editora: Bentham Science Publishers
Citação: Dias, Ricardo P. (2008) - Size fractionation by slalom chromatography and hydrodynamic chromatography. Recent Patents on Engineering. ISSN 1872-2121. 2:2, p. 95-103
Resumo: Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtained using low flow rates) and slalom chromatography (obtained using high flow rates) was reported in studies related with the migration behaviour of proteins, plasmid DNAs and double stranded DNAs. Slalom chromatography is mainly applied in the size-fractionation of large double stranded DNA fragments and may compete with electrophoretic techniques. In the present paper it is discussed the main patents, applications, advantages and drawbacks related to hydrodynamic chromatography and slalom chromatography, as well as some strategies that may improve the performance of these simple size-fractionation techniques.
Arbitragem científica: yes
URI: http://hdl.handle.net/10198/7011
ISSN: 1872-2121
Appears in Collections:DTQB - Artigos em Revistas Não Indexados ao ISI

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