Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/632
Title: Development and evaluation of a normal phase liquid chromatographic method for the determination of tocopherols and tocotrienols in walnuts
Author: Amaral, J.S.
Casal, Susana
Seabra, R.M.
Oliveira, M.B.P.P.
Keywords: Tocopherol
Tocotrienol
Normal phase liquid chromatography
Walnuts
Issue Date: 2005
Publisher: Taylor & Francis
Citation: Amaral, J.S.; Casal, S.; Seabra, R. M.; Oliveira, M.B.P.P. (2005) - Development and evaluation of a normal phase liquid chromatographic method for the determination of tocopherols and tocotrienols in walnuts. Journal of Liquid Chromatography and Related Technologies. ISSN 1082-6076. 28:5, p.785-795
Abstract: A high performance liquid chromatographic (HPLC) method for the determination of tocopherols and tocotrienols in walnut samples is described. The compounds were extracted with n-hexane, using a simple solid-liquid extraction procedure. Tocol was used as internal standard and BHT as anti-oxidant. The ehromatographic separation was achieved using an Inertsil 5 SI normal phase column operating with isocratic elution of n-hexane/1,4-dioxane (96.5:3.5, v/v), at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diodearray detector followed by a fluorescence detector. The detection limits were low, between 0.037 and 0.266 (jig/mL. The method was precise (% CV less than2.8%), accurate (% CV less than 5.6%), and, as a general rule, the recovery values were high (mean values ranging from 93.4% to 104%).
Peer review: yes
URI: http://hdl.handle.net/10198/632
ISSN: 1082-6076
Publisher Version: http://www.informaworld.com/smpp/title~content=t713597273~db=all
Appears in Collections:DTQB - Artigos em Revistas Indexados ao ISI/Scopus

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