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Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/5249

Título: Isolation and characterization by asymmetric PCR of the ENDO1G gene for Glucan endo-1,3-b-D-glucosidase in Phytophthora cinnamomi associated whit the ink disease of Castanea sativa Mill.
Autor: Meirinho, Sofia G.
Carvalho, Marisa
Dominguez, Ángel
Choupina, Altino
Palavras-chave: Phytophthora cinnamomi
Glucan endo-1,3-b-D-glucosidase
Asymmetric PCR
Issue Date: 2010
Editora: Tecpar
Citação: Meirinho, Sofia; Carvalho, Marisa; Dominguez, Angel; Choupina, Altino (2010) - Isolation and characterization by asymmetric PCR of the ENDO1G gene for Glucan endo-1,3-b-D-glucosidase in Phytophthora cinnamomi associated whit the ink disease of Castanea sativa Mill. Brazilian Archives of Biology and Technology. ISSN 1516-8913. 53:3, p. 513-518
Relatório da Série N.º: Vol.53,;n. 3:
Resumo: Ink disease is one of the most destructive diseases in Castanea sativa. The most common symptoms are root necrosies and a reduction in root growth, which invariably lead to the death of the trees. Phytophthora cinnamomi is an oomycete associated with this disease whose life cycle develops integrally in the soil. In the present work, was a fragment with 1231bp of the glucan endo-1,3-b-D-glucosidase gene obtained by amplification, using conserved primers and the full-length gene sequence by flanking this known sequence by asymmetric PCR. This fragment was obtained from genomic DNA of Phytophthora cinnamomi isolated in the European Regions of Castilla-Leon (Spain) and Trás-os-Montes (Portugal) and associated with the ink disease of Castanea sativa Mill.
Arbitragem científica: yes
URI: http://hdl.handle.net/10198/5249
ISSN: 1516-8913
Versão do Editor: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300003
Appears in Collections:BB - Artigos em Revistas Indexados ao ISI

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