Biblioteca Digital do Instituto Politécnico de Bragança   Instituto Politécnico de Bragança

Biblioteca Digital do IPB >
Escola Superior Agrária >
Biologia e Biotecnologia >
BB - Posters em Encontros Científicos Nacionais >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/4261

Título: Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi
Autor: Jorge, Lurdes
Dias, Teresa
Andrade, Maria
Vaz, Madalena
Dominguez, Ángel
Choupina, Altino
Palavras-chave: Phytophthora cinnamomi
Ink disease
Cytoskeleton
Actin
Issue Date: 2010
Citação: Jorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Ángel; Choupina, Altino (2010) - Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi. In XVII National Congress of Biochemistry. Porto
Resumo: Actins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural proteins, found in all eukaryotes. So, actin gene sequences are used as tools in scientific research, for example, for phylogenetic analysis. Actin in Phytophthora infestans is encoded by at least two genes, in contrast to unicellular and filamentous fungi (Candida albicans, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis and Filobasidiella neoformans) where there is a single gene. These genes (designated actA and actB) have been isolated from a genomic library of P. infestans. Phytophthora cinnamomi is a host-nonspecific, soilborne, pathogen of many plant species. In Portugal it is most important as a pathogen of chestnut trees. The purpose of this study was to clone and determine the phylogenetic retionships evidencided by Phytophthora cinnamomi actins. In order to isolate the actin genes, P. cinnamomi was grown in cellophane-PDA medium and genomic DNA was used as a template in PCR amplification reactions combining degenerate primers Act1, Act2, Act3 and Act4. PCR fragments were purified, cloned into pGEM-T vector and transformants were selected. Complete open reading frames (ORFs) of act1 and act2 genes were achieved by HE-TAIL PCR, and submitted to EMBL databases (Accession numbers AM412175.1 and AM412176.1). Act1 has an 1128bp ORF, encoding a deduced protein of 375aa and 41,972kDa. Act2 ORF has 1083bp and encodes a deduced protein of 360aa and 40,237kDa. Deduced amino acid sequences were analyzed using FASTA programs from EMBL databases. Act1 showed a 98.9% identity with P. melonis actB, 94.4% with P. megasperma actin and 96.0% with P. infestans actin2. Act2 showed a 98.9% identity with Pythium splendens actin and 98.6% with P. brassicae actinA.
Arbitragem científica: yes
URI: http://hdl.handle.net/10198/4261
Appears in Collections:BB - Posters em Encontros Científicos Nacionais

Files in This Item:

File Description SizeFormat
XVII CongBioq2010 comprovativo poster actina-2.pdf932,13 kBAdobe PDFView/Open

Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 


  © Instituto Politécnico de Bragança - Biblioteca Digital - Feedback - Statistics
  Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE