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dc.contributor.authorMartins, Ivone-
dc.contributor.authorMeirinho, Sofia G.-
dc.contributor.authorDias, Teresa-
dc.contributor.authorJorge, Lurdes-
dc.contributor.authorMartins, Fátima-
dc.contributor.authorChoupina, Altino-
dc.date.accessioned2011-02-14T09:55:10Z-
dc.date.available2011-02-14T09:55:10Z-
dc.date.issued2010-
dc.identifier.citationMartins, Ivone; Meirinho, Sofia; Dias, Teresa; Jorge, Lurdes; Martins, Fátima; Choupina, Altino (2010). Cloning and expression analysis of glucanase genes from Phytophthora cinnamomi. In 9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologia. Évorapor
dc.identifier.urihttp://hdl.handle.net/10198/3369-
dc.description.abstractPhytophthora cinnamomi is one among the most destructive species of Phytophthora associated to the decline of forestry, ornamental and fruit species. Associated with this oomycete is the ink disease of Castanea sativa Mill. Glucan endo-1,3-β-D-glucosidase catalyzes the hydrolysis of 1,3-β-D-glucoside linkages in callose, laminarin and several carbohydrates found in the cell wall of plants and fungi. It is generally thought that glucanases play a role in plant defence by digesting wall components of the fungal pathogen. In oomycetes, glucanases have been studied at biochemical level for their possible role in hyphal tip growth and branching, where there is thought to be a delicate balance between the cell wall synthesis and hydrolysis. Fungal cell wall degrading enzyme production is influenced by a number of factors including the type of strain, the culture conditions and substrate type. The aim of this work was the analysis of homologous expression, in P. cinnamomi, and heterologous expression, in Pichia pastoris, of the endo-1,3-β-D-glucosidase encoding gene ENDO1 produced by P. cinnamomi. The expression was studied during growth in different carbon sources and was also performed a time course of endo-1,3-β-D-glucosidase production. Different plasmids were used to clone the gene on each organism and we used RT-PCR analysis to examine its expression. The major expression levels occurred at the medium with glucose as carbon source. These and other results will be presented.por
dc.language.isoengpor
dc.publisherSociedade Portuguesa de Fitopatologiapor
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/67628/PTpor
dc.rightsopenAccesspor
dc.subjectCastanea sativa Mill.por
dc.subjectENDO1por
dc.subjectHomologous expressionpor
dc.subjectHeterologous expressionpor
dc.titleCloning and expression analysis of glucanase genes from Phytophthora cinnamomipor
dc.typeconferenceObjectpor
dc.peerreviewedyespor
degois.publication.firstPage181por
degois.publication.lastPage181por
degois.publication.locationÉvorapor
degois.publication.title9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologiapor
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