Biblioteca Digital do Instituto Politécnico de Bragança   Instituto Politécnico de Bragança

Biblioteca Digital do IPB >
Escola Superior Agrária >
Produção e Tecnologia Vegetal >
PTV - Artigos em Revistas Indexados ao ISI >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/3119

Título: Identification of predator-prey relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay
Autor: Santos, Sónia A.P.
Pereira, J.A.
Rodrigues, C.
Torres, L.
Pereira, A.M.N.
Nogueira, A.J.A.
Palavras-chave: Coccinellids
ELISA
Gut content analysis
Polyclonal antiserum
Predation
Saissetia oleae
Issue Date: 2009
Editora: Springer
Citação: Santos, S. A.; Pereira, J.A.; Rodrigues, C.; Torres, L.M.; Pereira, A.M.N.; Nogueira, A.J.A. (2009) - Identification of predator-prey relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay. Journal of Pest Science. ISSN 1612-4758. 82:2, p. 101-108
Resumo: A polyclonal antiserum (AS) was developed and characterized for the detection of immature stages of the black-scale, Saissetia oleae, in whole body homogenized field-collected coccinellid species, using an indirect enzyme-linked immunosorbent assay (ELISA). The indirect ELISA showed to be sensitive to the S. oleae AS, detecting a protein content between 0.118 and 0.0374 lg mL-1. The specificity of the ELISA was tested by assaying a range of sympatric predators and alternative preys with the S. oleae AS. Coccinellid larvae obtained the highest cross-reaction and a positive–negative threshold was established at 0.674 lg mL-1 protein. A total of 1,322 coccinellids were field-collected in three olive groves located in Tra´s-os- Montes (northeast of Portugal) by the beating technique and were analyzed to detect S. oleae proteins in their guts. Fieldcollected coccinellids which attained a S. oleae protein concentration equivalent higher than the threshold were considered as a positive reaction. In the overall collected coccinellids, 21.2% reacted positively with the S. oleae AS. Chilocorus bipustulatus and coccinellid larvae obtained the highest percentages of positives with 43.4 and 40.8%, respectively. The greatest frequency of positive responses occurred at the beginning of July, mid-August, and mid- October coinciding with the occurrence of the first, second and third instar nymphs of S. oleae, respectively. Thus, in this study, the role of coccinellids as natural control agents of S. oleae was highlighted by the number of individuals and species that tested positive for S. oleae AS.
Arbitragem científica: yes
URI: http://hdl.handle.net/10198/3119
ISSN: 1612-4758
1612-4766
Versão do Editor: http://www.springerlink.com/content/1612-4758/
Appears in Collections:PTV - Artigos em Revistas Indexados ao ISI

Files in This Item:

File Description SizeFormat
56.pdf314,05 kBAdobe PDFView/Open
Restrict Access. You can request a copy!
56 resumo.pdf259,24 kBAdobe PDFView/Open

Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 


  © Instituto Politécnico de Bragança - Biblioteca Digital - Feedback - Statistics
  Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE